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b. i e X U H U 2 ? I D C > @ = < E J A 5 < 3 A E 0 < 9 ; C 0 G @ E ; G ? 9 - 4 7 = > ; B D A 8 : 9 B C A @ 4 6 7 7 < , ) - 4 = @ # A B : ; 2 < , < . . . ; - 7 4 7 @ . - - $ / B 7 + 7 * + H A A : 1 9 1 ' : 3 7 : E 3 0 @ : 6 > 0 B @ L A < ( 9 C 4 B 4 8 2 X : V Z \ N e [ - F5A ^: s r e e F J L 6 Q A H D E U > 4 5 / A ? 2 > ; 3 C 8 3 B 2 : C 9 = A 8 ; 4 : 8 2 > G 1 < @ D 6 / / 0 B 5 C 3 8 1 / # ; < : 1 , , A ( 8 ? * ? . G ; = 9 > > B > ; U 7 6 4 ' < 4 4 D 2 H F < @ B S C = D = I 5 M I g ] _ t *Gee9$ s j ` T Y H G K 6 > & 0 C > / - . 3 I 5 @ ; 3 , ( ; 9 , L : < < > 8 - > ( : 1 1 E 9 3 B K P I D J G @ B 0 : 9 7 8 6 A C R D H , 8 L K = ; 1 8 : * - < 2 H I C = A E < 2 6 8 L ; = 1 < ' - C 9 B H G 8 A I R Q Y Y | y 8@>nyQ1 i g _ W d c m m ~ O| B- \2 c o Y R R @ H P H E < O N A ) 5 I 7 M R 6 9 I * T G C < D D G S J L > _ U J G [ L M W c u P%#>5*=`gunSlditLuvgp=T8K# s r \ V Y f d h q Mw}iV1 m n j W Z _ ^ H O Q f ~ t )c[7 ~ Z n g O T > K . > 8 5 9 D 7 H E 8 N E : 2 - C 8 ' F 8 B 4 . 6 + G : < $ O A P B A G 7 O 2 H : ) ' 8 = D @ U ? A 5 4 " = ; , 2 C = 8 B ; < 0 4 ? 7 ? 2 L @ = C R E > E 7 > T ^ n R b /YZ/ { [ _ Y i c r x 3VjF. s j { Q F M E I H 3 > H 8 C 4 ; G 3 = 7 7 B 4 8 ( 8 ? ; 9 2 C 9 " 2 B 6 : + 2 " ; B % & * / 2 L / E O S R i s s )UxlP 9a (!.(*;IJ91<. o^D k \ K S J C C = ? ! 7 C / 7 ; . 9 : 5 H : 2 ? D 6 6 6 9 7 A M ; 8 9 9 0 H 3 3 9 M 5 / 3 * . . 4 : 2 A 2 7 2 # 9 * 9 + + 9 , @ 9 C F 7 . 8 * + 6 K b I s 9N||T\- } H G W J E ; D G > 4 A 9 A : E 4 B * ' ? H 9 B 4 ? * , : 7 2 ; 8 7 8 A > - 1 8 / : 3 1 / 1 * 5 . 6 = . , 5 ) ? D < 1 , 4 # / 3 ) * & > 4 1 - ! < 1 B H + E 2 * 8 C 0 . 7 @ 5 ( 0 9 : D F F * ' 8 . ' 6 > / / 0 2 3 1 = 0 A = I B ? W > & W F < 2 K D A & ( < 8 L A . D 7 * : 0 ; < 8 " 4 ? . C 9 6 ! 7 ) , * 3 ; . B 6 O F ? J X N \ r p fj~eD ~ ^ o t b V Y W ~ s DZeN1 h d d K G > : C D 5 < > I 8 6 + ; - 4 ? 0 % ? < + % 8 > ; J L & 6 9 ? + 1 F O L Y ` r |
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7d24d9f2abf34e1cb737c3e13fc39b13Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_01-56_0549 3130Seq 12249 12249_Costion_K1357_matK_R jmadera 96-Well
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> x~ vV cfJ & 7 A Q p-4 ATTAATTATAACAATACGAAGGGGAGAATATACAGTATTATTAAGTTGGATACCTCATCATATGTTCACCGGGCCGTCCGGTGTAACGGTCTGAGGCGGATGTGTAGAAGGCTACATAAGGGCCTTACGAACCCGGACGAGGCCTTGGCGTCCAAGTGACGAACAATTGATTTCACCAAGTGGATTGTGTGGAGTATGGCTAAGACTCAGCTTGAGGTTTCAGAGGGGAGTGAAAGAGTTGAACCGACTTTAGAGTACAGGGATAAAGACGCTCATAAAAGGGAGACCTTTCCCGGATCAGAAAGGAAGAGAGCACGAGTTGCAGAGATTCCTTCGTTGAAAGAGAGTTCTCAGATGTGTAAGTCACCTTCAGGGGAAGGGGAAATTAGATCTTTACATAATCGAGGGGAGAATTGTATCCAAAGGAATGAGTAGTGACAATTGGTTATATATCACAGGAAAGAAGGAGGGATTATAGGAGCATTATGATGGAGGCAAGGTATTAAGATCCGACACATCATATTTAATATAAAATAAAACTTCAGAGGTATATACTCTAACGTGGGATTTTTCAGGATAAGATTATCATTTAAGAGGATAGTTGACTTAGAGCGGAGTGATAAATACTTATACTCTGGCACATGAGTAATATAGAGCACGAGTATTCTCGTATAATTCCCGGAGGGGGTATAGGTATATATATAGGTAGTATTATGCCTATACTTATAATATTATACTTATGGAGGGTTCATCATTATGTATGCGGAGGAGACATATCAAACATACAACAGCGGGAATGACTCCTATCTTATCTTCATATGGATCATAGAATATAATTATATATGTTACATATGTCAATGTGTGTGAGTAGGTCTTTCTCTTTCGGAAGGTATAGTGTGAGTAGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 03:27:23 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS U BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA + V$ DATA
+ V$ : DATA + V$ DATA + V$ / DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 3 LAST ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS =[ P1AM ` P1RL Y P1WD h P2AM v. P2BA }B P2RL o PBAS A PBAS = PCON VT PCON R PDMF PDMF =r PLOC K PLOC D PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% =S SCAN 3 SMED
SMLt SMPL SPAC AX] SPAC =k SPAC AX] SVER 4.0 SVER J SVER , , Scal @ Scan 3 TUBE G7 Tmpr < User phAR phCH = phDY big phQL c phTR phTR p This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS U BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA + V$ DATA
+ V$ : DATA + V$ DATA + V$ / DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / p GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 3 LAST ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS =[ P1AM ` P1RL Y P1WD h P2AM v. P2BA }B P2RL o PBAS A PBAS = PCON VT PCON R PDMF PDMF =r PLOC K PLOC D PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% =S SCAN 3 SMED
SMLt SMPL SPAC AX] SPAC =k SPAC AX] SVER 4.0 SVER J SVER , , Scal @ Scan 3 TUBE G7 Tmpr < User phAR phCH = phDY big phQL c phTR phTR -&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGUNMCp:K2O}MvrQ>(A#W7TrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9K5=aD<6s*;k1jk[K2;+hj3@*7_62Q$OEH5 G,90lVSFsA bFX97z?&q6X+9mK\TI_aEdO{7+VDTj.uFPqWgZ*me2&dAr&;t[W?*b1j glX ,cW`$~+hj:gh_)!hs|O1^M6C,nJ3qo8-@MV^A$+v/y#PzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=KubQ2a50jj,4o-8N'nsv&'Y?sxWlZN.YHng=! AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS U BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA + V$ DATA
+ V$ : DATA + V$ DATA + V$ / DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / p GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 3 LAST ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS =[ P1AM ` P1RL Y P1WD h P2AM v. P2BA }B P2RL o PBAS A PBAS = PCON VT PCON R PDMF PDMF =r PLOC K PLOC D PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% =S SCAN 3 SMED
SMLt SMPL SPAC AX] SPAC =k SPAC AX] SVER 4.0 SVER J SVER , , Scal @ Scan 3 TUBE G7 Tmpr < User phAR phCH = phDY big phQL c phTR phTR