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"3 <HHz <a <CH9:,6 y|vow [a]D*&"                   #(5"@8?@94<I;K5(*.,#    N",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR?\cgggggefdb`_]\ZXUSPNKIFC@>;8641.,t```VVVzzz,,,888CCCONNZYYdddnnnxww                                                                             <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6d9ff28d80504b41809f2d469b09d8c3Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_01-56_05493130Seq1224912249_Costion_K1357_matK_Rjmadera96-Well K1357_B4_RKB_3730_POP7_BDTv3.mobSeqPop7z#*/11112358;?DJQX_emv&5BO[fpx}}xpg\RH=1$|pf^YTQNKIHGFEDA?=<;;;:8779<@CDB><;<>@AA?>>?@@>:4.)'(*-1479984.(# !$(*++*)(&%$" !%''%! $&&$"!"$')'"!!     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"&$717d;/2F1*); ;h, 3:?)*,+*?)D5*2H$-;AM&& 7?IE=5_5M:9?<BFCO4/6XAAJDCF5HEH__&I5PIG8Y?rnHs<?^ZlYQLT]mrNaiN_<xU^<pNq~^eXR_xYTurrkLQIcif,|8egyn`JopaZjst[]pzjxc~E~zpu**5 `Q' ????O   @ $:"!#))DN)Vp%f^81>up(dZp.Ehp3xD e|hIobp<_ W(+fzdwYm7T>GK h`APdqr,p 4Chpg,g|Z^7`!d!`Q~< B,5bx8gH>p!i 4pk= -v=DMfHd$p,!pJGWf Jh8SpQ/'>i|2yU9p{cp]HpDgUp0pppppRppp)jp&*p%pplpXpApp)ppp@8p 9UZw%  Qg+KJFPx>= 4i )29%O#6%0 a&"cX>))&$F(#3'-  - ;)$+8E)/8!:*/ "1#      ' ## #& +0 .,:4+4  22(%'.# *+8@(!: $" $(;7 #!!)!/" " &#  !2/"!% 16)#!+( ( $#%,!/.!*!#("6(!4=d%E,*%"$ '2eF&(?4>?#D>% #!*4:$-;]t"_.W'! !"!%W7. .%$6 4-CWNai@*)Y-C;13%+C.03262!$&4 H6.@)2.+9A  'NVV(<D8G3,KO(D 7"%)2&!6J2G7[E*3"-M+=&9>5;`&CT $* Cr^\JPu#3BGtD3(WXO=j-hy3ELQ?-)W#!jYWzaMiLeA1.J5_aM>eXI3o8#n5Sx& __Q6fL-r`I_orlp\\Dnnv44-fTaV_mIlvrhm|" 2" !@}m^x_QB+ea5Br_(qqFxa|N6@\q.ED%nEN]+>SQd0UY<EQ-+@+Mlk-4IN%0RuK5B}5X5k'~9TcCTTTTTATCACTGGGGCGGGGAGATATACAATGTTATTAAGAGGGATACCTGCATATATACGTACGGAGCTGACCACTATAACAGGATCAGAGGGAGCCGTACCGAAGGGCATAACCGGAAGGAGGGGCGAAGCGACACCGTAAAGAGGAAGAGACGGTGCTTCCGAGAGAAGCATGGGTAGGTCGGGAGTCTCCCTCCTAAGGAGAGGGCACTGGGAGAGGGGTGGGGCCTCCATCAGGTGGGCGCGGAACGAAGGAGACAAGATGCGCACTCATGGAAGAGGATTCAGGGAGTCGAAAGGAAGGGAAGATGAGTAGCATATAGGGATCCCTTCCAGGAGAGGAGGGAAGTGTGGAGGAGACGTTCAGGGAGAGTGGGGATGATACCTTCAAAGACTTATCGGGAGGGACGTTTCCAGAGGATCGAGTAAAGAAAACTGATAAATTCTCAGACTAAAGGAGGGAAGGGCCAAGGACGATCACGGAGGTGGGTGGGGAGTTAAAACCCCCACCTGATCGAGAAGATATAAAAATTCATAGGTATATAGTTGCACTATGAGAAATTCAGGATAAGGTTATCATGAGAGAGGATAGTTGATTCCGAGGGAGAAGGATTGATAATCATACTCACACTCCTGAGGAGTATAGAAGGAGGAGTAGAGTCTGTTACCTCCACGTGGTGGAGGAGCAGCGTCACTCCGAGTAAGTGGAATTATGACAATATTTCTAATAACACACTCGTAGAGGGAGTATCATTATTTACAGGGAGAAGGAATATCGAATATTATATAGCGGAGAGTGAGCAACGATACCTACATATACGGTCTGGGATGTAATAATATATCTACACATTTCCATCTATGTGGGGTGGTCTTCTCTTCCGGAGGGTATAGTGTGAGTAGAGATCTATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 03:27:04 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +W2DATA +W2;DATA +W2'DATA +W2YDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~PLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISAP1AMeRP1RL^6P1WDlnP2AMzP2BAP2RLsPBASETPBASAPCONZPCONWPDMFPDMFAPLOCOPLOCHPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%ASCAN3SMED SMLtSMPL SPACAWHSPACASPACAWHSVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCHAphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +W2DATA +W2;DATA +W2'DATA +W2YDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab4FVoc#FWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~PLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISAP1AMeRP1RL^6P1WDlnP2AMzP2BAP2RLsPBASETPBASAPCONZPCONWPDMFPDMFAPLOCOPLOCHPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%ASCAN3SMED SMLtSMPL SPACAWHSPACASPACAWHSVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCHAphDYbigphQLcphTRphTR^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+@O5A Mg~9+)95=(2h.]^>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@f=r\~DYW1Mr03}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#