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"            ! $%                            $                                                                       N",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR?\cgggggefdb`_]\ZXUSPNKIFC@>;8641.,t```VVVzzz,,,888CCCONNZYYdddnnnxww                                                                             <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 212979a74c214b80b18e3fbc833976d9Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_01-56_05493130Seq1224912249_Costion_K1357_matK_Rjmadera96-Well K1357_A11_RKB_3730_POP7_BDTv3.mobSeqPop7zJ'V&i\2x-g+r . ^ 5 z F f l ^ F 3 . < Y    & , , * , 4 C S ` d ] I +  | t a 9 ; h Fw 4z(^04X)u(NxAWYCl1v2xC 9@fE%9Ri~ 'N@{K"q X`%-}\scM;,!&6J_tSc gs G} 1zG02D\u ">e7RxI\_4hI5+-8Jc^)# hOc` l--l~V</+/5=CHJHB:33?]#| Vm^)^Vz5/ZqQ6"1Ve4EI>&E,Z$V@fV/ -TLQmq^;$|.gHcohv%<Wx KC Tq &r7(h>P1mg-'!JAp [^ -SypT=+,Jz@lw\ ?*W5 %n7+[~Q*p8&sT8m?*7k9B]=jK. 0AIH<*  )=Vw+:*o3 #<Sdlnq{KKs61O4T3\NiN>1$ _m)<)H`S3I7 1a<o'^NrqtzRQ&O#cu8S"V#}h R6SG/vU)EE 6  $(,2=NcypZF;>S{4d|yePMmD9wiut2IL"idf>6 L&hJ958=?<3' #2?DA6%;dcNSZL" z7Q| SER5E&*p\J5   )045556:BKSVSH7$KReS!K%262( $/7<?BGP[hu~ykYD/ INgZ,P( Z/(8i"fcTtEf1 !7IV\YOB;Cfq"@)xD 'E_osm_OA99DVm+3"zABy$-jujPj^'Be>#A\r{vw~|eJ1 S<pk9h]^gqy~zn_M=59MpFekV)^MHIF>1"&3CVkw>Yk="7KY^YK8%,Q}aa{C":HZY6$  !)29<90# 8X~|E %Jp}T-2hah.     .{sSuTw0   %*(  =y'}]73W1Z\61A 1AIF;) 2StO~y?I !URoN{x \x*9`sT4 $++& <:S> ab'>R   1DPQMNaBU?!! 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"% -01FdF KI0$ Q4; 3Tc%OL!5YJN%%[D)!HW3c[ ]14@G6-HD+=H\a7=@.5P&Y,]7>D!=R-4\O=!_bK200 EV:2L R0W =T$. $;8+V79KSQ=KH!bX/2KRUJY 2MWY @b@Y 2)&J 6J=W%^CUJ[_LL; 1TUSJP5\ 8b<-0K\]?2FZ^bWL +9 FC*:I2*#,)3WGLF8aI+NcI/'c2a"`2'^MF/IRJ;)RBNAU f  ;6xZ nYGk *q7]Y.P NlrskKI0@ G&=p!5S :u>0t!h/ !{Cr>aey_b^CyS&?[,Ri^1 CI:13e,aJT"V5mrBed t Dk>rNpld`/7)'8v^A`YuzW9&'mgFv'%.4)k3{r3z" [ ]r=-FYz8t[ /  4S` @8KFf8 jrK?s[3+ o!S.Bw. ,L&QN  OO zrH&!D&>bqv9^!kx.%x?~Jlu;4u Oum/  * @j (Co1<$I_ T ]ig6bUJyl`8XW.Z=j $B)N.h+G/]()68953$  \+%1 # )''0V  Ff4 A >$, , :   V (Q;5D .? )@@B>"C   6#  M 3g?.5r ~*H5%F;V, 6@9'!-BA$3 !A:&A"(G7\CDHgt.  )k.08 #+C%2F0 H//<6uiiZKR!c?#MgH&%:[MO)F!!)!R!!F4W 4!!F%!F)FFFFE!!.&))$ F!!I**[H+4"&''e*K(m*.***Q*H:B/'W'P4GGC@AGGGG='02>>>>GIII1GG/&GG/>>W9>>IIOGG///>IKG>S(I>G/IU>>>G'>>>>>>4XGb1n ]*V{o|z71D576C1OL,`S4-X5W' !/h;Onnu  WH, X/=CNe?CB2T}QP""o6aY;FbW.;}Ovmu2d`p 4 KVqVJn)c(RMDo8|I_|ymR6<%,eQosU@]puFz[pB"s:z/.+~Njkdni(14 AdC N{\pL<wP5{*p5> Qwsgl<_/XA0fW&i1Fj;LZ^d 3z)I;q+\]w!7M$&VdW9vfe|i=U0#A?44Fw% {wY <V>Yk]dil_>_ujm DC)-: pXU 0W6RLBmSS4L$[cSB~KO,7#L5 8 { R: [GXAa^VHHIC&zL@v\P)[ kGb@\-MwQYo{ ~f|JS&cifBJ{vm==V6TUjzzHPo4yN;GeaJ|qgz~PRA"Y(x}r-Mm^S T5zrF@H5.T*t ]5 m1C?rTk XCAAAAATCATGCATAGATTCTATATGTAATGCCGAAGACGCAAACTTGTCCCTCAACACACTCATACCCCCCGAGCCCTACAGGGACCTTGGCTTTCACACGATGCAGTTTAAATAGCACACGTCTTTAACAAATATTAGCTTAACATGCATTATTCAAACTTGATTCCATGCTTTCTTGCATGGGTTAGCCTAATCAACTTACTTAGAGAGCATCCTTATACACTTCGCCACCGGTCTGTAGGTCGGAAAAACTCGAACTCCCCCTTTGTGCCATTGGTACACGTTTAAAAAAGTGAAGCGCCTAATCCAATAGTGTTAAAACCCTAGTGGACGCTATGTGCGCCAGGGATAGGTTCAAGTCTCTCTATAGTTACGATATGAGTATGAAGCAAGCTCCGTAAGAATGTATTTTATAGTCTACATCTATCACCTTTGTTAGTGTTATATCCCCTGCCGCCTGGGAAGGCCTTGCGAGTACGATTAGGGAGGTGAGCCCCTCAACTCTCCGTATCGCCACGGTATCCGGAGACAGCGTCGTAATGCGCGAACGATGCCCTGGAAAGTTCTAAACAGTATCTCTGGGTCACAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 03:28:03 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSbuBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA *TDATA *T8DATA *TDATA *TDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~`LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS4P1AMOL%P1RLOGP1WDOPP2AMOYP2BAOO^P2RLOUaPBASOO7^PBASOO5PCONOOE8PCONOOBPDMFPDMF4PLOCO>KPLOCO9PSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%4SCAN3SMED SMLtSMPL SPACA]SPAC4SPACA]SVER4.0SVER bjSVER,,Scal@Scan3TUBEA11Tmpr<UserphARphCH5 phDYbigphQLcphTRphTR!4This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSbuBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA *TDATA *T8DATA *TDATA *TDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabpFVocpFWO_GATCFeat00pGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~`LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS4P1AMOL%P1RLOGP1WDOPP2AMOYP2BAOO^P2RLOUaPBASOO7^PBASOO5PCONOOE8PCONOOBPDMFPDMF4PLOCO>KPLOCO9PSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%4SCAN3SMED SMLtSMPL SPACA]SPAC4SPACA]SVER4.0SVER bjSVER,,Scal@Scan3TUBEA11Tmpr<UserphARphCH5 phDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? 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