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? * 4 3 5 T 5 @ ) 5 B @ J 7 / , 2 1 8 , 5 $ 0 C 3 . 1 4 7 7 5 ! < , I 1 9 * 3 ( 8 2 ! A G - < L 2 2 0 5 @ 2 H I N 9 + > ' ' < . , + 4 % A 5 2 8 : 6 ? 6 8 9 8 1 I A 7 7 = > = ; ' N < L E F > 5 0 , 1 P # 7 3 ) D 3 9 / F $ G ! , . E # + 1 5 % 1 H L ? ) ) 4 ? E E @ / & # E < ; + 9 R 5 F 4 : 5 7 P F H E ? . 2 c c V C C D H A 3 E A Q J G S T ? M 4 8 I L % 5 6 P E T A 5 @ H . B ; B R # ? 9 > ? = ) ' O 3 5 : B 7 $ I ? # W J < A < 7 Q 5 [ T 5 4 1 7 7 [ 4 - . 4 0 6 A ) C 4 J % : ' < 7 = : = ( / 9 6 ? D : ) . 2 5 : + 2 4 G H 9 ? J N 8 1 B / L / < I G C 3 4 , G + 5 H C @ C . I G / 4 0 ; C / ( 2 0 % . H > 8 > P 3 J 0 $ N > ; M $ 8 = ( < F . < 5 < P ^ 5 < 9 ( ; 0 = 1 9 5 - A A @ A 9 H G G 0 9 2 ; 8 @ 9 ' ? @ % ! 2 ; @ : 6 @ 4 F E 1 H ? > I I 2 ; ? E : + : : , = ( ) E 1 , H 5 0 & 5 # / A @ 9 < + H 8 9 C * R > = J E 9 F B = D 9 F I 6 4 Z . C D I 5 & % = : G A 7 L > 9 F , 5 Y @ K @ L _ Q > 7 @ R F = G D G * 2 b ; M K = + 7 @ I L K F 0 @ S A P A H l I J R C , @ \ / I B J . , 1 + 7 O _ b R S C D E + 4 , E @ E B % 7 8 @ B F : L N G A 1 ? * 9 D B X W 5 3 4 7 9 L > > , E 9 3 O D J 7 F ( Q L 4 P Q : B > , P B G O > & D 6 : S R & T F 4 : E * E D H \ J > K [ I J 8 d B 4 3 J Q g / / T B : G 9 ; 9 E A 3 e A < ? I @ , ; < < @ 7 O A 7 8 7 5 1 L ) ? ) * * + : 3 I 2 < S H C E l c L ? T E R L G : g 8 ; N P 6 V > : I L L ) B K 6 Q F B W G D , / # 3 ; 1 3 : Z S 1 M 7 B = F = G L M T U 9 6 < ? C Q ; + J C F G ; ( = , 4 0 7 7 F 4 7 1 C L ' - ' 3 @ 1 < < & 5 ) 7 < 9 8 C [ M R 3 X [ 9 : C > R 4 @ F q B [ L F 5 ? 2 9 ) K R ? G V 6 ; D I B 9 X ? X 5 e Y S 8 ? F * D $ / 4 A B = E > / 3 : - F * : 8 6 X 7 R L [ F a D G Y P N N Y L 9 ? 8 B L W N I _ H , C M E A < F _ H 9 D U I G L S 0 Z P U H ; > E L 5 N ? : . B S \ E X : B - Z K < D B K @ V = * T ? 7 P U : Z T S H < = D B Q W 3 4 L B L D , N Q F M C S = @ F [ * Q = [ T 8 2 N 2 4 > I 2 D M > I I / ( > 5 * - 6 G Q E E X L 8 I E N N N P B H F K G @ 3 7 9 , + = % / F : G J - V I * 8 L R n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d ? Q J Z Q F E U D + F T 8 C G S : > M H C ; 7 9 X ? & D I M O : L < X W T 2 J L W F V D 9 D ? Q ^ L R S P W J O ? N p U C M I 1 5 P I 8 ; U F O ? O U J M R P = ; P b E D @ O F 7 M > ? B = 8 D D . A * K K N B U ] ] I Y T I d Z O ^ W O o V F F H \ I C = 8 B E H N 3 A = M 1 7 : . 9 ! J M . W X S > C 8 U ] > 7 C 7 Q / I B E O O X W M J ] 8 Z Y 9 8 G T F T P @ R L G J M w U ^ 7 8 M F J H E W M [ [ R @ ^ ^ K J K G Q S 9 @ K < @ G = V Q 6 K : P H N J 9 4 B / M > B % E C Q N A [ M L a Q I B ; > V S @ D = E X T D V ? N + J L < 6 < J K X : B V U Q S C ` P M S H R I E G N N L ] > C O @ > G , = ` S D a F H ; W ? 5 < M \ G Q L 1 B 6 I 5 K Z ; I + @ K : = @ ) B T E A = M 5 D 3 7 q G @ J L . F J 3 G Y K U 6 7 c : f Q D / F g Q , & E ) 8 Y V E N M S ` 4 M K G 2 & < ? 3 1 ; V N > 9 8 @ C < A = ; > K @ ; > Q E M 4 P H 6 M I O J , < ? 3 H ? @ L C 4 H @ : = ; > D 6 J M ? X V ? V E H \ K U H < J H E G M 9 + W 3 : B 9 T W > B 2 = 5 4 E 3 4 S P > B N E H - B K ? O K J E 0 = G ; @ Z H W F a Z E 8 L K < G 8 ] ` L P 6 J 2 7 D I L / / W B N M h M O U B = R / 1 3 W C L S J F M - ] Z : ; 7 I I L G @ P L A F N $ , V 6 : 5 E F T * ? 5 7 N P M D @ L D < G 1 O X 6 F % 7 5 0 9 ; C G 7 9 I A 6 0 @ M L D 5 V L G : S H C P D < < F < Y M B C > P J @ 8 T L = S P L O A = Q < B Y 1 G T W S O B / 5 G C A @ 9 H K T A 0 = = M 6 " B : > D d ^ g > L P J N I 3 D V R 3 7 ; 1 X ? 9 ? B G 2 B 7 L J ) I 3 8 H = D M ) / 8 Q ; 1 > 1 M ) B 1 = L 5 L E ? G c > U , 5 M C : R H M S > < ] R D M V d : L = [ T ? 8 R : C @ < < R 6 + N 6 R I S I 9 K K L ` J E O ; = A 7 ? J R A < ? J ^ 4 H 7 ? 8 A 2 E B > 6 1 2 4 G - E 7 G M Y A 4 F 6 I 6 C d \ A L f S A 0 F H 5 ; V U T D A < X K - J M ; 7 b 7 B 8 G @ , F ; < @ X C 9 / S M A A P ` M L V t N = N E P P S D F D @ W < T P G _ N H B : 7 L B M H _ W P A H U C [ b h J > X V P J U @ : ^ N L G 5 T Q M > R . ` K O P K Q N J A ; R A M g ? G 4 O E P W j O U X U ; C @ 5 E H 5 > @ Y ? W _ N ; K O ; Q A Z G @ < F J I C E P G Q ? I C 4 K D < W ^ Q L G M H T G > 8 ? M F C T < B Z < L G I N 8 E ; A L U U J 8 = < G > @ 9 Z ? E > A 5 Q P Y U E j = F Z L ? L N 9 J ? L _ \ Y X E D T O B P H : 0 I K H T = ? 7 J 2 N N R : G B J ? J h S S @ X Q S @ T = 9 F Q M M V Q D ; Q > C 0 - ; I 8 D : I G D b R S P ; Z : D E T B ; M Q I B 5 > E I A @ : C V K E L ; Z Q C C E N G M E U \ H J Q V N 9 L . 7 > ? S 2 7 5 K 3 D L > O W ? Q 5 A E N M F Q J 6 B ^ H [ ] D S J M P D 4 U 7 B H A W b M C K ? 5 Y H S D K G K G K ? 4 8 G ? B B O E J G ; < P T L Y Q 5 6 = K ^ W F F K L f M O E D 7 F 3 K J P 9 5 E \ > ? > 4 A < E 0 ^ B V Y W I _ _ T A T = D 8 = B > 0 Z 3 B Q T A F P Q C D O P @ N ? M @ T E : I ? - 7 Z G K O 8 H ? 7 W 9 C E F ) ? F P 6 / ? N , I G G > D Y ^ 2 X 7 H @ 6 A $ H E = T I < S < ( 4 K E ] P * D 4 L @ 6 S R b P \ [ U T L P Y A b F : E I L J = 1 T ! G K = A D > Y Q H H > > L X Q C d 7 < G D V U W G Q N M X T J L b g ^ V L C H X c 6 Z E : V E T 8 J Y N P > p N 4 S 8 = 9 1 1 Q R ] E 6 : & H O \ ? S K Z @ G Q Y N C L H \ W F K A U @ A @ C N N Z R T J R T C 2 Z E E E P R : ^ I 6 C ` H V < K H < W T S H H R E Q R a N Q : a < 9 ^ ^ S k W P Z Y = Y l P R @ < L 2 D C @ M E < 5 @ > < V G U K 1 G _ D ; D I P 5 g S M M H H O 6 < C a 0 Q Q I \ : S T F J H < < ? X h Y C Z ? V [ U V M R H J < L A N e M Y Y Z J T Q O _ P 3 M J c L m Y K U 9 B L ] F A N X i U W > G \ J H J E h R R P ^ 7 H 9 H M N V 8 B N L G N Q R ] 9 S I ; G N R Z R N M 1 K A X E N L f _ a : ? 4 K [ S L > J d E < U ^ ? W R K J K A - E G ? F = < B < 0 ] I F [ = a 5 J T d T e H _ C _ Q W _ R U a ^ d 5 G H W E F ^ J P ` O Q R Q S ? * O L [ G I A V 9 W Q Q J Y d > e ? Y L g B F 6 / < ] T \ P b O C F i M R S > 8 ` N 0 Z ? > ? = D E @ R 2 R L V N _ C J J V > Q K U 6 L _ b Z J d G C < A e 4 Z c Z : : P a a O S E [ ^ H ] I K R F B I ` b I F : ; E [ T G L V N 8 ; V k @ J S f 2 U F 4 b X ? B B O X < ; K f J W U V I F T A H G c R C Y c V \ R J L l @ Q E Z V f X b f 9 S G y \ U H \ h H L b c ^ @ Z _ S O U d W C X P R X B L X F F Y R F K [ A Z L Y F L L M U U F , M @ T Q K S C K V R W f D B F B @ D > O L ] K _ \ V U V W W V f E M ? Y S Q Z H I X Z ] t J V K D [ j T C F d W C T ? V \ P I S O L Q S a F R X 0 e Y C K B A I 7 = K S N @ K J P C Q M K S E a I K - - A P ; L D @ R c 1 C P G P P K = = 2 W I E = F A 0 P J _ X H > ? U 7 R E H M D E L R < ? \ c M A U I C > ? O ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f5d0b362b9d24e5a8ac4076b7a1c94f5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-20_09-08_0530 3130Seq 12248 12248_Costion_K1357_matK_F jmadera 96-Well K1357_H7KB_3730_POP7_BDTv3.mobSeqPop7z b vE=Ki \]JN(8 z
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-20 10:56:37 -04:00AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Ǿ Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab
FVoc FWO_ GATC Feat . . GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Ǿ Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH phDY big phQL c phTR phTR !q*D]{SmP{QJHeXSbNfP>A0&&e`r/zapM.=!@D:Z>K>D`Vd96M8@/|thLG^r@uPDsvA;DmwC&Y?^dIrLei;(F}K7T]Zj7y!R}*Z8y0Pajeoi@LaiY2]C0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4\KUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^bAmK7^d}|}{B.@k5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQEO=4g `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H6DQcjR'QDb^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0