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F N ` e Y C 1 L Q j R A R k o e P a f l ` h W a Q f f ~ q \ _ e B 7 4 A M D A A 6 F - * 0 , / 0 6 8 ( - 2 7 U @ 5 S 9 G + 7 M 9 7 < F I P a ] j X S ` O S N k % 2L v U _ W S Z [ [ h N U U j n | x P P D Q E 7 < ? . R T L < 4 N V = 0 D H D Q G E R y b @ R [ n ] c 2A- #!?; @. l l t r W h b X R J [ W t n d A P M R F 4 0 + > + 6 A J F ; ) : O 4 1 9 D B > 6 7 F 6 3 - . M Q & > F I ; 6 ) P 9 5 1 = G I I O 5 @ 4 ( M Q 1 X F P [ : l { r b U { V \ v S ` Q j d s y | w R _ Y a h t q e u q t 4!* v d ] d F Z 9 Q [ V f G b X i I f _ L G G m w GI4I3" $"%A& z e i U j l Z S P O L ; J 5 W > < 8 Z S R G R N I J X o r y t n r \ s e o \ f n \ Y h c w h S V _ G Q R h A S F 9 N ^ ; 3 T 6 E 6 , J > 6 J B * M X 1 > M 0 K B > - / D P 5 5 . Q X 8 < V 8 ^ Y . K K I - $ : > : H 6 9 = 8 X A S Z U c S T 6 R o p } b v } ^ W K R J C @ K Q O B E E @ ? ; " P A G 9 < > = ) B - 0 & 0 / 4 + D Y 4 9 4 I 1 % K 6 M , 5 $ ' , - ; 4 : + @ [ B 7 = 6 O R D E E O X > I @ B N D ~ d l u { u o P H E < / ; 8 ? F + @ 1 G 9 E X : . B 8 ^ 4 V U > R * E 1 H M 3 J ` C O H P ] y o V p V f Q h l h m X U P o x p ] O > ) C Q D 2 9 ( J 6 I N 9 & # : ; A < % > ) J 6 . 0 ( E A ) < > < < . R ; ; , : M @ 7 9 F # 7 B ? G . F 5 , : M J @ ? ' I @ F 4 C B D 8 = 3 : = F S J b ] A L g a } | e c O Z Y k ^ _ O a i l d g y r [ K E ? K H ; K , E / - < 0 D . 8 = D 3 8 B C H I G G 7 3 & 0 J > 0 H 1 7 7 . J H E ) = D ' Z ; 5 0 : V U O S W Q ] M e u X y | o f d ^ N P j r j ~ r g d p d u v Z o i z i Y 9 K P : 4 > @ O R 0 - 5 D G 6 R = + E S A X ] 7 O ^ \ S Y P Q v i + "6(1+ '3 l { e j r v @ W _ d F h v w [ V \ L C < M H A = A 4 @ 2 ? ; P 5 2 6 > 9 + L M D - m Q Z g T d e g R d d i g l w h a a _ p Y q b n Z _ k j i x r { h Z a p e ~ [ r k o e { ] \ = M H D F 1 . C + % : * 7 - I 8 D E D K C M A H D N 9 ; K T < @ ; N A P L g g i c m ^ v y ^ u ? n R e t j | q i J j a J L O % B C K F B J F < e R E L K X W j | ~ m ] e i s a X L L I = P : N E O 1 N S 7 F ? S U a K w t s { b u b [ T G T ; F 3 P 9 J ' 3 I ; . : - ( ; \ G 4 > 3 T : ; \ m Q M U ` T s [ s q ] _ \ k _ [ 2 Q A \ R ^ 4 G R P k F U l M ` R ` i ` w o x a U V Y S B ; K < W 1 H 7 > 1 = @ F ) & 7 = > 2 6 @ ) 5 - ) 7 3 , B & M E 4 8 = 7 7 K 5 E : 0 ? : A E ; 5 C 6 G : / 6 8 H . Y U > I J @ > P g V S s x p { m a L U R f _ : ; B X 6 3 Q 3 M > < 3 > 3 , J D O < 7 B & H # K = M I < 2 * ; + J 0 $ ? D 1 3 / = : X 5 * F P D = ] H ^ [ E F Q ? L R q F j V f ` X { i ; ] ^ B S J E ? + T 5 " : 9 1 K B $ 8 ' & ( 6 ; * > F ( R ' C 8 = ' # @ V Z E c ` V W T V _ b s u y v c Y D [ K J - L 0 Z = @ A c T T = N f M G F Z b e t l { b l X F e L @ O X 7 K , / H . 3 ) = < ? . . ( 7 $ 7 = = - @ ; ( D 8 K 1 X N 8 , < = C @ 9 = : 0 T J / C . @ H I ? = < 3 X ^ Y F Q D W x i u c N X Y N W ? C C G = I V C e r N f U e l e x l b ^ j k e p w d ` m a e U D D V C h 0 : ^ G P R R ? < C [ V h ^ v o r s l | m j q r h ` t I H W I H J J P * . G H G G J E > P M > / > k J H Q < S Z W [ f j d k ~ z w | y i ^ W N O J U G P [ M 9 K X V R V E H T ] Z \ k c h l o r x g g v p e \ X ^ _ N A [ k _ e k l } ~ a Y e h k L g i A } t q W u b s ^ I Q b X Z V 0 Y ; Q # 4 A + 2 . 1 4 2 2 0 / Q 3 g / T P > 9 8 A = - L 4 L Q E Q ) ( C 9 1 A F J Q C % < C ; : > = [ R T e r k d t { r s d Z [ ` ^ 5 < X W I I F B I I > 1 B / 9 ; 4 < R @ ] ] [ P @ J H d U g f c a t ^ F 9 V K ; > O J S I K 3 N N ^ G O E G ? J Q F F A / , J R : E @ 2 C 3 6 \ 5 6 L = ) 3 ? J / H M 4 A 0 = T J I M 3 7 / - ; ? N B 7 " > H S c L N f P r f i p } Y Y ` f S o L E ( ^ S W I @ O ` K R G H L K ? 8 9 Z N 6 ; : I 3 J C 8 < 9 A e = * ; D 0 8 A K B = W U J T K b Y _ m j u o { y u a | ` m O ] c X J I G Y L T U M o V W X q o j O t i y ] o h g I u o i | x v y h q V { d \ b g m N \ q [ x l u x { [ c m Y [ U 7 = : 2 T K O M G = ) J A 4 G _ [ P S @ T U E L S c W a w s c ` n ~ v s b [ ` M N Q [ ? G Y R r Q f f T ^ _ Y ^ c h ~ ~ u s b k Y P q v l { { r g ` [ j a g r k U z y y o b X m S O J E 2 A S @ @ 4 8 . > N G N 1 B Z < P 1 N H I J J C " : E C E Z F O 3 N U L L i C H W ^ : W Z j ^ Z e t } n { v k f c H [ I 6 C 9 W I : I O > ? Q 9 E E Z f m h w j a k W d W s { S w G e ^ [ u ^ } p y o t o e c j R j i @ U Y K N z W c b - A K ] L Z V O P W B Y ; N N _ R I N W E Q E H F L i > M W G Z h w l t { t } q ~ w c o h N d ; D V e Z X K x q z u x V o a m [ S P < Y M C G T < C L I > G L a P D V I ] g B M G F ] O Y ^ & ^ I [ V C G ; L U Z ? Z @ H 2 K [ Q H ; ? J D ? V ] I B 4 @ W L @ 2 P W p L I Q e g R h i _ b x | y z r d a a b ` ` D X \ B O - d a V ] c R T c e p q l i u n h t x k e q j T \ l j w r m \ f m a R K ` f ` T S ` 5 7 M @ O : X ` g W Y l i u ~ v s v w a p L \ a k f b T J " J ? B F * U L / M K B 9 M X C C 6 B S Q B U C ] F > 2 @ G C R K \ N : _ d < < J 7 O L X R 4 X I 9 Q H i S C 7 Q G B J b j L d Q V i [ ` Y { u y z | G v c \ f X u s r y | ~ y ^ \ g Z K V I ` O D T L @ 8 > E < Z L S H J Q + 9 P O T 0 E d X Q Z E : f N W < N M T _ R 9 $ N D W Q X \ E J P N N J S F I ; K T Q _ N n V R { f f { v x v x ~ t w k X _ y g y y } } i v c ` S ? @ V N B ? W O L O ` B E 2 R J [ O B X R T O D 2 H c F O K M X i ^ ` | j | y z ~ ^ v i r v i V n b ] | v | i ~ y l t T Z R ^ ] 6 Y X P Z ? F K Z M S R g V h ~ e { ~ j Y | { } y } y n w | t s ~ } s j e \ m t | ` U ; K e V h m l g X V g p g V u ~ v ~ e q j b e O Y s [ ` N O X Q V d A I Z ] ] P e k i _ a m o l s } x q \ W R L Z H \ x Q ` \ P t n a l c w n n X ` Z f L k G ^ @ _ _ T 9 W j ^ M 1 O T T S I W ] a \ J F L ^ O Z T W e c v s o u s ] X y x ~ ~ s { p o v ~ ~ Z a { p z z e X a s N R : [ M > ] b P 7 E s C G O L 9 J I 3 N * O W @ T B U ^ X I Y \ F n d V j a ` l i | w Z ~ | z w } e | y x { r i m v ] d d V c W Q p t v n m z x q r z | d w u \ e n R l d S k S Z F T H c l Y r d J Y _ Z Q N f b U U m J R J y M H ] O _ p Z d l h ^ r u { { } { p y e | N g h d \ o V C R C U b ^ X j a w k c X k r h ~ | r M r p ~ Y g ^ m y h x l | v t { n f z | ^ X k d S R D E O e K P V b _ ` D N J \ W D - [ U Y E d u ` i H m [ E ] F [ k Q K Y S L g L \ c a M \ T V [ M b F ] ^ ] W a Q B W H B P N [ D Z < a ` ` V Y ] c g d a T Z M j v b K X H R ? V X M f ^ [ n W \ L b g [ \ W F e J O D c E h h k P Z h o h ] s u Q x } z ~ o e { R | m s ~ { v v y y | y m u ] e k d W a T P 5 _ ` X x b o K S M O L q a \ r \ i ] E ] Z c [ v y [ Y 9 s c Z b o G n e n k m Y Z t [ P c b M \ M X S 5 9 @ v X ^ \ ^ c N n f h \ s ] X X j n ^ g f i | a x ~ e x ~ f { u v t e n x } } c M p m w c k Z d c b v U n r l } p } u e S x O ` n g _ \ e U d [ T k X Y I i \ d o q o h l y } 2EhNgUhp=/" w q h s x w o { f i k o v s u s q x j v l m o ` | o q n u n g e r v ^ w e Z o N w w k u T v s e i r | w t x k a { ` f k c b ] ` ` \ U s [ X T [ t S ~ S o { L [ a Q m i Z _ n [ L Z j o ^ i V o d T j c H ^ u X N G u ] U V a ] T J \ e ` i j Q @ Y i h T T V @ ^ P e g p U W [ u d d Y c k j [ O ^ l i e d F f ^ _ j @ b S g M i p B ] P i b X 7 P Y i N U W I S C M X W \ N j k U T Q W f b Y Q Z ^ d I a O i P I W a h W P k W X S Y R e j d d \ U a T I @ i S S ^ ` \ X O P ] L [ V ` J L S U J P V N ^ G _ R Q i J N X N c G O O a R l X X H W _ e j I ` b G R F n g J V R w r S = D L d Q Z ` Z a e \ e c \ e ] o [ _ T O R d a ] d b ] ^ Y S K a K S a V F L j \ [ a i [ a J Z V S 4 F X S L J M a N W e ^ d E H F U @ \ \ m X i J T Y L W K V V ] k ` ] X P g F Z Z W M ] d L ` [ < S L a R B U [ W ` Y \ ] W M \ P F P ` 5 v U @ V Z D R Q T I j ` Q F m M a W j N b _ M [ I L [ N
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 76964e4486d7425b86ee2cd3b8cfd3a9Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-20_09-08_0530 3130Seq 12248 12248_Costion_K1357_matK_F jmadera 96-Well K1357_G6KB_3730_POP7_BDTv3.mobSeqPop7z 0ANVZ^biotslaWRWdv|fXU]hniV9 >cGNJ h V hQ;# ,Hau)l=xoD^ KV _3 iI+ | g V K F D A : 0 & # ( , - - - 0 6 < A A > 9 5 5 9 ? F K N P S W ^ c f c \ Q G @ = > A E I N W f { )6:5( k V I F H L N J B : 3 2 5 : > @ @ = 9 5 0 * # ) 4 = B C @ > < ; : 6 / #
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KB 1.4.1.82018-04-20 10:56:25 -04:00AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN ASPF ASPt x ASPt x B1Pt w B1Pt w BCTS V BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA . ] } DATA
. ] J DATA . ] DATA . ] DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS b P1AM P1RL P1WD P2AM 8 P2BA F P2RL * PBAS fO PBAS b PCON {y PCON w PDMF ] PDMF b PLOC p PLOC i PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
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. ] J DATA . ] DATA . ] DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / q GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS b P1AM P1RL P1WD P2AM 8 P2BA F P2RL * PBAS fO PBAS b PCON {y PCON w PDMF ] PDMF b PLOC p PLOC i PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
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7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% b SCAN 3 SMED
SMLt SMPL T SPAC AQ SPAC b SPAC AQ SVER 4.0 SVER K SVER , , Ǿ Scal @ Scan 3 TUBE G6 Tmpr < User phAR phCH b phDY big phQL c phTR phTR _R|!{VmMm[*&`cz"B``,_bX@sWFa}TYKt9>,3HB! Z c4K_f].r47'3QbIiEx~h6dYH.l_pD8Jq,./b _|(L{2TVyLyuiCgGz^n3HPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'21{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*MP4;Z4Lh2d?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!Kp8m|HtfpE}Ou/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LU?_fv(h'e(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>