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T Y T e A ^ Q F D K U O [ g a 7 L K Q N Y W R Y M O ^ A M L k E [ Z G R V X L T P M O a j ^ ` G m R W B X g p F J S M Y Z J e M F Z F D v L \ d V N ^ L U ` h S L L P a O Z _ G Q e n Y V [ _ U V F F C \ P P Q \ W j M [ V U P ] [ _ [ g Z ^ I d k \ R ] e T P O W X V W F H I [ V a I U \ @ M { V R Z R ] F Q J W U R Y X O ^ [ K D a X [ X p _ W ] d G S J M b U k U S P j B L [ \ K W ^ P U h l j M O e V L ] W \ r b 4 Q ^ [ N a K ` V d F \ P L ` U L Y e S [ b a e Y U Y j Z \ Y r e Z ; O Z i M \ P \ W P ` ] [ b Y V e z S ` r ^ N w Y L _ O Y g e q X T l O S T i _ > \ V W b T ` Z d A ] r K M L T Z Y W P b P I c d K L ^ b W W H J Z I ; V S X Y N _ a P Z I @ ` P _ Y i P Z _ U a S O R J M _ f f P a X O g k V H I \ _ P b U l L S S U N d U E ] ] ` V p T [ Z K b I L ^ Z _ a N R S w c V H I _ G [ S N N [ X R \ M Z Y ] a ] \ Y P ^ Z Z Z q [ T c l t X g X ] J ] a Z W X Z _ [ J U S ` O ; K F Y ` _ Q a N c ` X _ _ a W P \ p R \ N f K \ [ X H Z T S h ` C - M i W g ] d U O ` e U Z T R S N [ ` H U h O P U V L ` k \ J l M ` c ^ ] W J M U R O _ j u R i H T S ` W W V W i i o U [ ] [ Y J ^ M n [ e ] Z Q d Z L B G T G _ ? X U Q K M Y S d P Z e I U s O = Y F ` L ^ V c ` [ e N ] L P d Z g ^ M Y d R Y Y N i T ^ Q Z Y n Z \ f V ^ c [ j V \ I K _ D R O S L U N R _ m b ] h \ J \ M N a R f \ ^ d S X i V \ W W ^ \ H h \ G Q a < U c ^ ^ j J V M S R R ] X O O F N j \ d g M [ m _ E e N J ^ P ` _ b n e c i H [ R d M O P ` L y U L F N L a Y J b ` ` X O W I S K [ T U X S U W O I D S p P ] W Z O L ] ] V ] b X c Y U W ` N _ J d ] ^ _ k N h X O _ W X V Z U ? c u \ O a T h c a = L Y N R D ^ < E _ L F U O P T T W Y X G Z ^ D Z H ] b Q [ ] L ` ` E A W < J X X _ T [ C E \ Q k j H a T _ ^ W _ ^ T \ a Y R N b J T c _ K T l S D g S F F O C X M W O @ T 6 m L r d ^ Q X i h B T Z U I ] i Q M a N G F ^ R ? K Y Q g T ] @ C O X _ X X h ` ^ c V \ [ V E K Q E X ] Q [ N M Q D 6 \ b [ V U ` ^ S P d J N V F Y T O I a J > J c j O Q W Z G \ T V U A l P f U L \ @ [ S i N ; X N Z Q D c Z V f G 4 A ^ @ ] L E [ I c G R W ` J N A f b S R X \ D _ l x I P N ] Y Z J Y ? H G K \ N C P V Y \ d T f U k d X Y K X V G A k r ] O ; ` a J P W R g R s ; ] J n O a I M B V X a F _ Z a H M W i c : d E W n O \ A Q ^ K R D U V ^ f ; [ ] P Y O P G K _ 7 N ` J _ \ f K G B X A [ R K P M L Y ] Y O U ; n H ^ a X O a Y ` @ _ [ \ ] O W N D f Z T T W K P ; 3 Q B K J r ? P T T Z P X d J L c B S G ] J [ [ P U i T W P X L G 9 G V r M _ \ [ S ^ f L L L O U b W V N D K D U Z K W y H Z F U O Q a E f M N O P Y R W i ] L C L G H Z - T U R a b f U J f R Q d < Q = 8 I Z O F M ^ C X U V W M E S M < d [ Z V X _ O a b A X c K C C V V V M h s d I W M F Q M b V M B P R d [ H D N Q ? X W ^ L q F X T X I R J V W ] Z ` \ @ Y o X Q O T b B i T P X M R h h ^ S _ Z ` P U T f V H ] [ N U O V - U < f V G [ U . u U ^ y P G X O i Y b X Y m P M @ N U e Q M N T f O b ? M ? ` E Q W a Q R P P O N c i X A d G = h D R W Z U S T Z V X s W H i X i U P B h a N j S G d D n [ Y \ [ T ] Z S ` k [ j V c T r c U X X Q f H T O l B T V S c q N P H F T Y ] _ Q S ] V X Q U ? k P P b O Q A T U M I i \ y [ [ ] Z P a J Z [ L S U ` K W T q d W V Z V W Q Y a Y G T ^ I k E V ` b R N : F W a ^ N O j h Z Z Q P H W = \ b ? 8 E c ] > P ] K f c \ G [ _ Y ] Z U X Z R T U I g _ Y e ^ D d = O B ` W _ N Q E R P Z U Z _ c N J a D V K Y X L s W U H R Q V T N N k H R o W O M a g V i U _ ] o T \ T c b V J P ? Y _ W J f [ F R ` N G M L @ V ^ l M b c c g P F E Z > [ h T J X N J M Z g ^ S X O j H P U ^ I ; f S P U Y H U Z T c H s ] c V Z X S [ . A L B ? S U I Q R X > S @ b E _ a [ J F U T G f K ; C = g M O ] P _ e Z R i ` M P [ G ` l Y b A ` L U F ^ Z X M ` ] X r d O X T l b J ? U I ^ W Q ` V Y ? L W R Z O g e V b R [ W j X U T P F d C Q J Q P A F W J U _ < T [ ] Q @ Z < X K M L X S \ Q b V X k U D X ^ N V F X s a X _ b ` H I ` X I p L q a _ g L A M ? \ S X b ^ N b N b N R h f Q V i N W _ N N U e N H c N W ] 7 H L W t o O Y O D W X N D L N S w ` g S N O O G M J b H P W M I [ ? [ N [ J S W Z T L T I A _ v h ] J ^ K C Y V T V H W P S E ^ F W [ b M L i H Z T J F [ L d m G I _ D G h T ^ P Z M e N h 7 \ i p Q _ h H T E S U V O [ N h Z f G P ` a [ [ ] X S n C V Y I L Y \ ] h a Q S K _ U [ j T Q L Y R c R V b Q V O B P Q U Z i T T B J ] \ R J ` N Y L [ \ Z C Z ` Z X T @ Q O ^ G Y J R H R b S V d E [ m G U X j \ ] R P S N ^ j E Q I U G Z : o ^ T _ N M b f l X n T d ] a I U R W Y \ S [ W F U ] M U _ b ] X O N T B j U ^ \ P a ^ S c L [ S I E W I Z Y N V a M G V c J ? S I N p O p N m ` _ Y c V [ : [ Q e M T d H K ` Y ` a c O T Q K Z Z O S M W N S \ j c b C P P j < h X ` b = G f ` \ P P j P m ? j h k N P T V Q H [ L P X R f C L ? M X R \ _ U M \ G d L N ] X Q Q M W O S P S L Q R R O = V g g R T W ] [ U Y c d X c [ Q L R K i D [ L D Q E ^ G b P X P P X H P I U D _ M Q T Y R b I R Q L j : \ V L T G C U Y S ` M M L J R R Y K R L ^ Q J _ d F B < H Y 4 W X A Q I H M W W S ` b T Y _ Z T H K X c ^ s V Y L Y Z F ] i P < ? Z V B Y d ] Q J K O K f F \ ] ^ I \ E J L E U Q V M Z b M U Y E L V a 9 V P 6 M D l R T W l c L U b H C b M A n V [ L ] b \ H R [ _ H h V H S [ l I @ [ Y [ ^ L e a _ M E W H S V d _ U T S N N S T D J L [ X J j W \ b ? G R o N @ P > X A > m G b X [ b X L X X m C N B ^ D S d @ N Y ] S M W X ` E ` K T N \ Z f b h c X L g D ? E [ I X G T S E O [ I ] l ^ Z ` [ J J P P M R V ^ Q \ P Q g J E b W _ U O U S h B G R Q M G P e W B P Z X N P h N Y M [ Y Z J D G H U O h E > N L F L S = D b L e i I \ N J _ V = A Z N S J Y _ O U M B ^ ] O E S ? I Q P U U S g S U e Q S V Y c c [ I G Y @ [ M R L P X T Q W > V Y ] T R R @ D H ] T C K U a I \ F U V M R E S V O S Q R _ K _ a a L X I L 7 O S [ e Q H ? c c P R W C Z L a I ^ Z D P ^ \ U H S R ] S f V _ Q Z X Y a M V J U c L C [ O G \ Z J G W M G S g b O V W A ^ L l V U V ` h D Z U R h F H _ [ h h U M G K N g N R L X J U P T Q S c H X e H _ c M H H W a K S K E A Y b O Q i D [ 5 q E M X [ C Q K j O U Y F ` [ d ` F W ` < Z L U E I H [ = B B Q Y i l @ T Q 8 g P ^ Q C @ d R H O ` H a > O Y R ? V E D M O [ [ U Z _ J Z T H R Y W T c V X D y ` T I = T Q M E = e X i U N j R R e H R d K ? W Z G O = D T X V f J @ c K c K f ^ K P \ B H U T S K X O ` p S g h V N L Z Z P Z I C K c ; _ U X e X L W M H B M Q Z B S \ J R N I U T M J u Q S [ l R I 1 J L Y R N ^ b J c Q Y I c m [ M Z W R X M X Z ^ ^ O i k = O ^ V Z X R G Z ^ Q n M Q A c R ^ ` J G n = d e W N N ^ N c W N O U M T E S I T K P [ G Y \ \ U ] : d \ Y L W P P Q l S ] a @ J L b U [ I S S R c S ^ a O M K M [ k ] G F W _ L S ^ W @ W c X W L P Q O [ Y f R N R X S ] ? b d U N _ K ] s T T A @ c W ^ 7 e Z L ^ 5 S V W A Q L \ i C W Z : 2 9 K c \ _ A R Y Q Q T ^ X ? V W q k S Q e ] Q ^ \ V S F Z ? C J 9 [ B L P B X P c Y R C K [ S S D R X C _ P E R Q g P J A K J K Z k W X \ \ e a _ R Z C < Z R R ^ Q 9 V I V @ V C Q J N ] d O ; G R W e L ; X I V ] Q S U h Z N D K S U L Q C L Y < ^ R L I W S Y V J V N G U N ? ] G 1 _ O = Z P Q c K j J B X < U I F C q A K B M M P F I E H O S @ C S ` b V f J T T D H b P X L G ^ [ Z Z M N D _ O P H Q T ` H T ; R b a M E i l \ Q Q k O Q j \ W [ H I V M B V ? ? V H Y J ] G I F J M V J b F [ H S S O O Q U O \ D I W M ] a L [ S Y I \ X W D L P G U a S R b K E Y a ] G T R J B L E H S \ K V R b A S = H F ^ M Z A : I P E N O P K ? H K [ W L 1 J D ] L T > P R W I S A - G [ ] c @ F X L O Q ` G U F [ T T U L L X J C _ 7 Y Z D \ C R V G H V N : I a W C O L S B W R @ J l D C V M ] L T 9 L [ Y B ^ G R G H J N a U a Q B M M V T @ Q W Z H [ N G K V Q T W E H L ; 9 U X X O I N V b E O 1 H I U L J M [ X L M R ` Y X V Q D T X L Y P L b F K Y Q L 6 T [ K I r \ h O Z M 9 O T L L I Y X R S F \ M M R S \ D P Q O E D Q f V a D L G Q E L X Y M f B V W W H 5 G T 6 ^ ] [ f N V ] U I A U C X G W E N X K D ^ K R O \ N \ T f L b T K U b N E I K d E ^ M Y U f I o I @ M ] b L S \ S K P E M Q N a = T U F E R T F ? X [ W L f d L O X P ` V R S ^ D Z N @ W [ S F h E K Q P _ W 5 @ U V O O G f [ B _ _ ` R H J N : 8 J M 9 M S [ T b T H V f I A ^ b R 7 L M X I [ C D ^ L T I M O O 5 _ c Z L V m Q : ] a B S O S P ` N N K V i L C T m S ] ] Z V W [ Y d ? O F Z T E Y _ R G _ P 9 C B R V T S Z : X E _ S Y T O c O j O S R E ] e I S V k J I T V C L Q b R W I M a ] X I n > ; X I E l O B K S c P U R C : F k H ^ E O P ] A H S > O Z Z J O ` L P V D R ? K H : ] U Y R A V Q O V J Q R a \ F U > J Y ] M H / U Q L M W J \ D 3 b \ V _ = ; @ I G V ] K T D ] F P S b K L O \ L G ^ J V Z R O 9 Y T = L Q X a 7 X R V G N X X T U Z N T U M J ` b a \ H < = I C A R S c X H Y V 7 L C [ V b d X R G X I I V B [ U \ _ Q S V b J ; U F L F C ; X d D I P I H e D I C W ` b P V C H H : D K M ^ J [ Q ? D P b M r D J R I L ? = @ B U ? [ U > H L 8 N b e C k O X U > T [ ^ R d U T Q J @ E Y u b R R Q > E R _ X S Q N X c J [ m V H B P 3 T K [ P S X S L : U = ? O P ; = S [ P a V L S Z h T S T M I M G L X ; C I E R J W Y U U A C g A O G H F X N O P c I M X O N h M _ M : U X a C ` K E U [ N J P L X B J ^ T : P F 7 Q F G c Y N @ V P T H I c J < T O _ G A H I Q L F _ Y = V P U G P \ _ ^ R H d W O M : Z ` \ _ G S O D R \ O F S Q O 9 F V U J W D D N D S E Z ^ b m M Y F E Q d N P W F L Y J Q ] Y V @ Z M b ] X U ` J S Z Z \ Y I h d U L = ` B Y P F \ ^ N G P ^ W G Q B c Z H \ > = Y [ \ ] F E f ^ m ; S > K H N Y T I ? ] > S ? V J \ ] S _ U S H N G B 8 N H K _ J U J Y n V U A A b ? Q d : T K E H e S T P D . I a P T p Z Y T L @ U f Q M G H a K G H Y G F O M N B U T S Z D d : T e Q G E W P L N Y R S I Q Z F X S V L Y W Z E J Z \ J J D F O T R N Y : ] R S O \ I O R E b ? S F D Z 4 Y B L F S > H R R F M N C 9 a g ? b S M ` Z W Z X a d P ^ \ X g \ M L Q G O ] Y I Z J @ N 2 E I O > L N L A H q X Q T S F V l J ^ N Q N T h 4 M G ] P H M @ L S Y H J @ c X Q K W W W O F B R I V P P Q i N Q M [ N = K O L K G g O \ ( X S : G : a J \ M K L K M U < X ? b P C d H V M i F U M G Y R b j > W H : Y [ X [ Q U b Y O A ^ V J U P S M ^ C Q G L Z G U p > G [ L P O Z e N = V @ H I M _ ? K K ` L Z b H M L O ? J F R Y X \ e W M S C K G V E Q E Y V T Q H h B Y W M Y ] U U I D T d > M _ > T P 6 k K S M g S U X a V X O 0 C S 5 _ W Y f = B N R S a R b W X d H S ; S @ @ P L H R = @ E H U N Y J U V W E G ` M S ^ [ K H R K W Z S R R = a O b n k ] S \ T C K X B L K S N G m F I Q ] S A ^ S G Y R ] L P \ ? Q W > T O T C ^ e B Z O S b N I V J c T Y 4 d V L ? O X ; U Q \ E C S E ^ \ C V : B W H > S L N [ b _ Y Q N [ Q N O S a G M ] T G X R Y P e L d R U U E [ R Q H W K C K _ Y 9 U ` L O Y [ N D G Z A I R T Z N Y P G L X d N M Q R _ P [ [ ? Q W c ` E ` K Z Z W a [ V V W F 2 X E R R W J a Y H S M \ N P F R F a . B C N P ? A U D T l O T M W ^ Y J Q u T G O I k N C ] _ T S P O Z F P U N 6 G V Z H L J O Y f \ J L O V I \ b J J R G 9 K : Q O \ \ V d W G d c H I Y M U Q _ ? E V B H M V 4 P c Z 3 G D Y Q < ^ T = E @ R M @ I = [ E T G S V = D J ` K @ a F G X Y W E Q V W S H D S A U S U l L N Z K K X Y G Q L M h P U O F G C c 9 M R > C S d Q Y R \ P ^ d O O V M O O J D c W V M E ` ; A Q L V N
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6a711d5a7fad4fd996b1113504fa9072Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-20_09-08_0530 3130Seq 12248 12248_Costion_K1357_matK_F jmadera 96-Well K1357_E2KB_3730_POP7_BDTv3.mobSeqPop7zJMaE~.S RP*
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-20 10:55:58 -04:00AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Ǿ Scal @ Scan 3 TUBE E2 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab
FVoc FWO_ GATC Feat . . GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
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