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  ]]]SSSvvv   ###///::9EEEPOOZZYdccmmmwvv              !!!"""####$$$%%%&&&&''''((())))****++++,,,,----....////0000111112222333334444455555666667777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 210b104d778249ea89756819e409ecc9Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-20_09-08_05303130Seq1224812248_Costion_K1357_matK_Fjmadera96-WellK1357_C3KB_3730_POP7_BDTv3.mobSeqPop7z]\[[]clv~{{{}~ulgglt{~{si`[Z]`b`[TONQW]`^YRNLMMKGA<:=CGGB:2--17<>=:768;>ABBA?>==<:8669<ACDCA@AFKPSTSSV\dnuwuogaaix(6>@=7/(" .ATclonmnqrnaN6 }yvuwz~wj_VQQSWZ]]][YVRMHFFHLNMG>4,*+058851.+**)))*+,./011/-)&#! 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"&3-- ./"&.. .  %#" ...   0% )          4/<( T1;DT#%=?/@[;M?LO4!O%+DY:>8!]1&9V,/,^6IObC "\YMc'ENS-\Qb[(J7 E!2]3I/.N^V!*ZCXK34C\`=YS'YK>$$d3^aHUM6VPa7F  40F(=: K":>!>IH;!W\&%* T& 14RI,`OU<7b cQ?1Aa !L"1 0;?H.:`8JH/-`!- 6:ZA+::X%*M4+/I8K@)6W R0]^; B' <FBBd;M7TD18D\PD#!PU.<$_%QDN;^PbJWCO1G+T ,/ NAB !$B0R05;H:*/$WC` %ZQXb\-VS!>=B .Td6H/**"FD :[B&!ba7 8*7K!.>]$W:? `K9&9G[6O\' W V0B!V@ 1 +W "Q H(P)GDY'X=a c$%I# dZME:W D^C!FM(@A0T;+$;K`L!QAH7SU(-@8] >^O#DV%7Q& 8M A<([2 Z1: ), YWC/ K >^2WTNO0 bcXP2S=$K"ZR57#'UMb^;b+bT &"'D=(`7G3K9FK2[0404! /$=*)N0 22R`R2`\2= 0TR^1b(W b&-$6 BL2?%cN1W ?%<L2caA?%B]2c ca2YaB=^ 22S*E2S%F,R]22)2V-7VAC2!2LPE6#G^+IQ2EJN* 6L/S62W]bZ2 ZPMQ=%JW+MPS "P*-X>"9PE`4R]PK Hc3FTN\$`6h##{A%M&2eLV|D-7=}~~VE6Kef<Fd":Y+>,F9'AZl8g]pV %ohAUt))Eu0^eS#P!]K<'3gHe9S6HD}/3xBAn8_|zq`>K Uv  zG:b=CV7d UOP:X3Q#Rz)[}MFp{%V`|]t 4  g{ -RiBzS n$_ ^E{?[}?} " <Wa 78~*I}LMP^rkh1tM"4\<qH,Q/w Q< \({l9GUA;4i?_&8QJ[UmV"G[yVyeF}kYp\t9I?xwv!bY b}uKWR~h?NVs=C?34%^`CxsB 367Dv%HE:^0EbBZTVi"%ik9WyiG~,E~LX>ud9VQJ[N]DupR;:)Bo;il!M=h,/t:kTs-  9>4  mC}@9UxIB%jDO2Kg2 -%_.hS 'sJSJlI`)V]E 3O) { >0 4[ 'kzvGSCln tX DHUm1n& uml_Z gs.@4IF KS)m9.7?C8.,:F*; -#;"%8 "(                          "  0 (4 6% *)  -!+#!-- ]8'JB?...3.!::+ "4U2D $&:@/,;0,KBRP,++"F/5'Q*,UU+%V9Z3)M&8Y4:C^33HF0 KKKKDKKKKKOOiiUiGGBrbT;ehLCEuX?^WR[xee\p6cge}\MgUZooooexi]hhh`G`L`kZmw{fcdru"G.rvg&mKz%y_x3!!!!!B#!!N!!!$777911799*)79999999(9o7iiLili>llLllll1lil,S>ql>>iii>iiliiL$lZlLLlll%_Llilli>il>ilsl' Hn F# Tppp>p7pptppFx aC0KJU{REp+>p_  ;-Jprs3wA)@ZQB8},uwdY_I"U5]|-6p^)qXE Sl3i DIf .s14!=B (#={+ 0B%N2?A - ,#%"$"/!2,H%! & 3 !"00' *;-0-<&    1  #2v8J"L%7-$      '!"$+ * ")C.)2/&0  $ 1  *! -(/(-'&,@a&$A1+Y!- !"-,%33&!-60&P*$1lK#['FaLX:6R;-53% :-7=?G,-AM0 N;9&$6*:4(<,-?; ,%:JGJ_3N%6 ==T+j2:P(E:w8O1vS8D5MAG?([A69ADJ59Es-J'JQE`dM6,Qs',%nu8;IC=z/GSX1"VQAEE.VJ?TZ9m7z =CJ@hQ@oP?:.jHyJ!}d<"|:)cnE9S<zqp30rl @d9E }Z1]Q0 m=$S0vY)21CvPxsU_2L ^CtumxvtLXHV>O5J_v'b{jOp7f1SvAEs8S V$51 8&oV_Wb'Jnc?d/ :m4^ dK"/OFvJATTATTTCCCATTGATAGTAATAACACAGATCCAATAAATGACCTATAGTACGGCAGGGCAATACCGACCTGCTGGTAACCTAAAACCCCTTCCACCATGCAATGAACAATAACCTTTCTCTCCATAAATAAAACAGGTCCATCTACAAGGGTAGTGGTAACATGGTTAAGTCAACGCTTCGGAGTGGTCTAGATTTGTACCCTCGGGAGGGAATTGCATAGCAGAAACTGTCCTCCACGGATGGTGAGGAAGAGGGAGAGGCCGTCTAAGGACACGTCGTCAGGGACGATCCTCAGAAAGAAGAATGACATCATGCAGAATCAATCGTGAGCGCACAGACAAAGGTGGGGTCAGAGGGCACACTCGAGAGCGGAGGGAAAAAAAACAGGAGAATCGGTAAGTCCTCGAATAGGCCCTCGCACAATGTGCGCGATAACGAATTTTTTTAACTCTTTCCTCGAAACCCACTTAACCTCTTATGGATGTAAAGGTACAGGGGCGTAGATAAATCCCGCCCTATTACCGATACCGGCCGTCTCCATCGTAGACTATTCATATGTATCTAATAACGTGTACTTCAAATAAACTCGTATGTATCGCACGAGAATAATTATGTACTCAGTCGGCGGGACGTAACGTATTCGAGAGTACTCAGATCTTAGATACTCCAACAATAAGGTACTCTACAACATTATGACTAATACTTCTCATCCTCGAACAGTGAATGGATAGGTAAAGTATATCATATGACACCTTATAATAATGCGGATAGGTACAGCGAAATAACATAGTGTTATAATATCTAGCTACGTTGGCATGTATGTAGGTATCCCTCACAATATCAGAGTATATTCTCGCGGCACAGAGAATGTCTCGGGTGGTATATACACTACATCACTCGGGAAATAAGATATGGGGGCCATTCGCCAAAACACCTTCACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-20 10:56:16 -04:00AEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTSnBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA -&ZL}DATA -&ZLFDATA -&ZLDATA -&ZLaDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOISUP1AM^zP1RL^s`P1WD^P2AM^P2BA6P2RL^zPBASYPBASUPCONoPCONlPDMF]PDMFUPLOC^dPLOC^]FPSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%USCAN3SMED SMLtSMPL TSPACAQ)SPACUSPACAQ)SVER4.0SVER cSVER,,ǾScal@Scan3TUBEC3Tmpr<UserphARphCHUphDYbigphQLcphTRphTR SThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTSnBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA -&ZL}DATA -&ZLFDATA -&ZLDATA -&ZLaDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOISUP1AM^zP1RL^s`P1WD^P2AM^P2BA6P2RL^zPBASYPBASUPCONoPCONlPDMF]PDMFUPLOC^dPLOC^]FPSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%USCAN3SMED SMLtSMPL TSPACAQ)SPACUSPACAQ)SVER4.0SVER cSVER,,ǾScal@Scan3TUBEC3Tmpr<UserphARphCHUphDYbigphQLcphTRphTRn(_T+D(_7&OMb'~6/lD-EkOptpuD:\E+mH_a2TS7}_'A\3[}k@_aA+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\dxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]4UvI{OF?jp,V0Id4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oX$^(`])>}JcVGkB*Sig]r;a4g0_[YBPxrl@;Gc+[b`[.b=jrOCy~R^bfFc':Z^xDFU)T;w>xP=O_l;mqy=Y`ge#cme/^it>QG/PM6MMJO LW~Pb@))O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#t[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}nz:z5>vQSdW79ZYdj8WoQK5F1_;T,V2%4oNYkk?[V\3291mor/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@