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F F > B 8 = * H < 2 H > K 3 C > ; ( = @ 8 9 _ A 0 0 6 > ; 8 H / 5 3 P > 6 J B A 8 3 M * 7 B 3 . / + E < ) A C & K 0 ; V K K < , 9 F G ? < 9 / # 2 6 D " : M F G ? E U D F ? A = M M : H J ] d T G I @ = M 4 : J 7 M = M H < B U G 9 H > @ E F : 5 S M N . N F / # B D ; M < " M C B 7 D D = L R H L L = \ P I J C Q V ; U H O K ] I I L T R E M @ S Y 7 k ? ; : K ? G S J Y D [ O H ` P Q @ Q . ; @ J - + F 0 Q M = U M 7 F F < 1 L 1 G = I 7 , - J 2 ? ; ] ^ 4 > > L L 3 : H 2 4 ; 1 W X A R + : J 9 F _ B ; M [ & U I W D g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` = B A U 9 7 / V R & F B C L Q @ B Y . C > ) S 4 N 2 B Q , G J F L C ' 9 O 7 I A 8 / 4 6 A K < H 8 @ 3 A @ 7 N D / 4 1 B J 9 B < = < A P > 9 = A 7 H M Q C K 9 ; U C P R 4 ( 0 G < C @ # K T H G M G F E ; C B F ; G ; ? ? G D U > E 1 > > ; J : L < 9 > G W C / ? 9 ? D @ 9 ' 8 8 A ) 8 J A / R C 8 < \ E < L ; 5 ; H ; K 8 1 b 9 K 5 M W B ` < = F L I U A 7 d E J 0 4 / > 4 > @ L 9 / 4 @ 4 R D G L N % C K H < 0 G B , 0 4 M * > @ 8 C 4 A A 6 4 * = 2 7 8 = 9 F I S [ t Z k I ? A C B 0 > A = E L R M < A O U 6 = B N F = 7 O G ? > L X C L R I P E A 3 G ] H 2 P P > : B O T D 6 G G J H 2 ; ; J K 9 > M R I M R I @ Y c L V < ? P [ K @ J > N M L E A ? A 1 N E 8 T E a H A S K U J W P 8 : H ? K Z S ; @ b U 9 H H E O B @ 2 H Z U Z V O Q ^ N H X l Y Q K W O l X N ? J D T W D L P Z F J F 9 ? > 3 ( B @ @ V F Z D E A ? Q Q > R 2 ? 4 G Q R K G E @ > b V G W K J J B J Z B L V L I ? < n M G B : 5 V O B D 7 A < I K k 3 3 7 K P N G : H N 8 O P @ = I : B K M L 6 R I A K F V U O W ' D 1 U W = X 4 > ] _ D Z L T R J B J . M = ^ K ] F ; T C R a E B S Q K S J B a ? ? = Y R S A N 6 D E 9 8 B \ H Z B X X F Z M ; G T R ; J F D 7 E : / 9 r [ L F Y I ? O P G H D @ 6 A F > F A 5 1 + 1 _ 5 + M _ / 8 < < B E 8 _ ) Q @ G 3 Y @ F @ 6 K V V G a 7 ; Q T B E B F F V G / [ U S X g G E C ] \ W A < Y 0 1 F ; 3 1 C D 8 O P / 4 D H 9 E G f J S R S C I @ L K C J I < O / " ? : < < @ B ? J S I 9 C I M J M N C 7 D > H L ? B B : ( C N G 8 7 G 8 C : @ N E 8 @ 8 ; M 5 9 ; ? @ @ ? W - G Q A D V \ ; H 8 Q J M < A > [ Z Z I = C K G $ < D K P 3 F : ? : ; : - H _ L 7 C F Z D A V A 2 L R S R 9 C D j S F ^ J T X 6 U P X 0 3 8 b n X @ E : L I A Z h 0 < ? @ M 5 G R 4 7 P Q C 6 6 A I H = ? 2 8 N - L H 7 3 - D H : M @ 3 + U C N = C 7 T > $ ) I 9 S 9 J K I N = Z [ 7 N ? X T G U D 2 : ? D . X I R 7 @ K G ? M K H 2 ( S J ] < ; @ 1 ' 5 H > 1 M 9 ? 6 G D > F > 5 M < E N P < b P Y Z A A M @ < F 9 > U > A J E S F J : > N 9 > > G C @ / C I 3 M 3 E N E G F X J B C H ; G A 6 = C : 7 \ W ; , ' N I O R I M H 9 S G O = L V P E Q 2 > I P L = 8 M Z O C P L C L U 4 F T F 0 L H P V J X & F = G > = A @ 9 G > V F R C _ P D X a ' N K A A B 3 > ( Y B U P > 8 . R ` T H i E ] G V U C ; F O ^ 7 X M F @ ; \ S M - ; J S Q P - , S B B = D ] R N C F 9 ; : D W R @ F L ? ? C K J N F 6 T = L B e F < \ I T 7 V / < U L R R P M D W > C C N N @ H I E K Q E V : Q [ = 9 C G ; \ K C L = F F > F H G U C U K d X M 9 T 9 X X ? B H < X S = R O = 9 I @ X C K R 5 2 O B Q C R ] V I A 2 G L 8 7 ( G J M @ B X E 8 Z E Z L 3 I ^ : \ Y = L B ; @ G Q Q I D t J ^ M @ @ G D L C J L A O P ? P > U < B L G S J A P B I I \ J D @ I C J ] F A V * : B Y Y L , I G C a Y I 9 L G Q P @ I 6 : ) 7 < ; U > J J L 6 P N X K D Q C L R I B [ S D > O C E < ? A : : G ; R T F F H @ H B 5 N V . 8 ? ? T Q X > S H 8 P K P P Q [ G I D 8 ? L Q 1 4 6 U , Q P M ? S : A 6 E 8 L D E U _ O L ] > E T ; P E A G @ G N ( C Y W E _ M 8 A B 9 E = H V F A J [ B X d N e < = K P * Y E I L R ^ W V Y h U G K U d P E 4 U W ? Z O O G 3 u A 3 R C I F I L @ F U E \ i D K N G Q H V T < ? N T : N C G L C H F : : @ N I A ? & 9 K 1 < B 5 < N [ W ; , ? 9 0 8 J V B K B B I D Q C K H M d K W D E R J D a U ^ N 4 = J I ? 7 - R G _ C = 9 D U A C 0 B X G L . H F O I D F B > $ N X R R F D E : 9 E K ; 3 N @ G F J ? E ) ; @ < ` 9 = 8 E * D R B @ M V R < W Q Q N ^ V I M P P A O = L 7 * L L T R < P 9 D F = 8 : U H M K G < Y 3 < ? ^ D R L G G E S G F F V X P K D h D F G F X ` F K 6 F I 0 = i F G Q J H 2 H ` U , & B A @ D 2 8 6 2 ; D 5 R = I 6 D - T D = = M A L / 6 P V S ; P = T S V X a : P E L @ H S ; C @ R I L F K 9 4 B 4 U D 9 V I - I 5 P < K F 9 ? E _ P J I N ] S Z X K 8 C Z E S E b 8 ? ^ @ G W 8 X = P D 4 3 W Y @ B J h H W V L P < L ] n H R @ T B T 8 ; I S e T D T ; _ d ] D P M H U / > M U A 0 F 5 L X N L C J Q D C > V @ T Z I O P ? d H 6 H I U ? Q G f V L _ U > E @ Z T Q G R O U R S I Q H 5 Y K R b E \ 3 : W E W Z U A c 5 9 E P R L 9 K G L ; M P O K A Q O J ] 3 O ] Y 4 P ] ^ N J Q T U G L F ? K V [ H I X B A F C U J C = Z I U < J 1 R 0 ( @ ] J X V K ] ] S E D 5 U S G O G V K V K _ [ D S Y G D & 7 N C I T F Q A C P ) K B : S O b B A J K Q P O J > S _ 9 L T @ I ; X L 9 = ` 8 T P _ A \ N > H \ G B 8 ) 0 B R U @ < O T < W 3 < S C F \ W N : ^ F Q G I I E > E P ? Y E V = > L @ @ ; S M Y g N S A R W B Y 4 ] I R ; ; Z U W R = R 7 = B b [ C A T S ? Q G Y ; ; G Z ; Y \ L 9 O < 3 < D V A A X B K < A A L P Q F G R E L A O / : W L E P V E N 4 M H _ R N ^ X I O h A ^ M S V H \ C b H B Y \ ? W C _ F T V ] \ S Q ` > ; Z < C [ K E M ] U K 1 N J I G y = O = S L C K I ; J I O K L ` U L H O Y Z Y < S V Y F W < J A K F @ A J P J L X - D Z U X d ? K Q H I U N I M Z W R F 5 : L Z O T ` Q C L ` G K @ C G \ H B : 5 3 a K W E B B > = ? C = 9 W F V S < D G 6 A E < I O Z = X S 1 : J Q X [ J @ Q 8 L G S ; C H V 2 8 c A ; > 1 \ E > C A H ] H T 5 K L I E 6 N O \ W O P B 5 G ; P , O E _ T F M = : ? N 8 C ] E C M H L e I > B 5 N A N H ^ _ B H M F N ` d l [ ; K 5 _ Q h Z B b S C R 9 G J ; F J @ K ? 5 H L [ ? ^ B R I G < A 6 + J R R D ? D = T R H ? 4 P < s b F O C : E A H W i b Y T L @ @ Q Z U Y > O A D @ a L L I _ M - W B J R C F C _ P M V L W I N E M d I T 5 I X D ] V S 9 ; V f J B ? * K > V T F W @ S L j N U # ] A 9 * A E V ? O O Q Y Y 9 P C A J ? ; U G b 8 d T F T Q H G J V E I K T V G W M E B I O B U S 7 J P @ 9 F V = A Q a D W ? B I P F > 0 9 Q k < O M Z ] = L H W c Z e 6 V @ P I : R \ H B 1 ] C : : U F 1 I N V H 9 D B T H C N ` < Z g A C I f ? L R > C R F J O 4 T N E U B R 9 6 S @ R : O P R E \ E J = Q 8 C K X X l Y Y [ K R K b ? I = Q P A Z J [ P h @ Q U T I < V Z S H C 0 B Z g U H Z J M L ^ R T H T O P Q ` T U [ \ < @ A O 6 b K ` S e F W V P L c ] p \ ` W H L P ] f T F \ i L a J ? > A G \ G M f U V A " Y @ b I M d R S U ; c W K M H U V ] P b O B U P _ m T h q ` _ a e e l c \ M _ U V U l J X _ Q J F J Q O K W d G O M @ ] D K K O U \ d ` Q U X d V Z V i W Z ^ ? R Y U R I a W M B N Q K S ? I a S D L C P I Z < U E N [ c 9 5 N A R P B S G ` H H \ X G L \ E k M N T O L ] g a O P ` T I Q M R ] M O F O T [ P M W F P A T [ a k \ V S ] @ U c g D R U q O Q L a 9 ^ W d K T e Q l U F a J c f S J ] [ s W [ \ ` H k h V ^ q j M P Z ^ [ _ J W Q C Q W b n U ` C ] X W E N ] > ; D [ X : Z b ; P _ k T P g Q T J Q R U S I L Q R C e c ^ [ G J Z ` \ Z U e P 9 o X N ^ V p W S Q P S U 2 J @ H A D C O E S 6 a V Z : M C Q O F O J S D F 9 5 S 1 V ` M D Q U 7 ^ \ ^ _ a 6 N 7 H H L e J A > G j Z N T O B X I I F S i = M ] P m \ d \ M g _ ` K Q J N \ Q T g Q T Q ` R V Q Q m J F > > b F V \ > 7 ; Z n D M Y ) Q G ] K K O T P Y 2 \ N W T V ] S N W R ] T D < > t _ B W f U f M ] N P Q Q L J S [ [ C I N C ] n U L R X A Y L \ h ` [ U b b Z ] X [ > Z m W c V D K U K a d H G I p Y j [ _ _ T n b d f [ K Q S Q [ U c X X K ^ Y S M M [ ] Q Q D O T X ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f287ec684623491abf449b952141d8f5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-20_09-08_0530 3130Seq 12248 12248_Costion_K1357_matK_F jmadera 96-Well K1357_B7KB_3730_POP7_BDTv3.mobSeqPop7z%J P "$?% %&'()+B,.13t579:;%; ::$99;988t765O32k1/-,)'%#"> { }aipn Y4 _ '"#%'}()*+- .p/1/1242 122435q654
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-20 10:56:49 -04:00AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Ǿ Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab
FVoc FWO_ GATC Feat . . GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Ǿ Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH phDY big phQL c phTR phTR vTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+%3N,;ByYn,C[_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rTssBSX?vz\HSF{,ComMOx8yT,I)_|_n?_;?oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAC?-4$&"Sf^wKB]A\9I*$6elkgahH;M[=g;^{ AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN v ASPF ASPt ASPt AUDT ` ` ' B1Pt B1Pt BCTS
BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab
FVoc FWO_ GATC Feat . . GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ ' LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
P2BA " P2RL
PBAS PBAS PCON PCON PDMF ] PDMF PLOC
PLOC
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Ǿ Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH phDY big phQL c phTR phTR