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( # ! ( 7 ( ) / / " 6 $ # + ' % $ ' , 9 ) 5 0 ( . ! 6 + # 9 , 2 ! - , # # ! + & ! $ / ( & > : 7 E / & ) - - 9 ) ? ! 0 @ % B ' # * 0 $ ' ( 1 ( 5 ( 1 " * + " ? " - ) % + + # 5 ' 2 / & $ ' !
, ) " ' ( # 9 1 - $ . ( * $ # ' 0 / & & & 1 1 * N L N v i O N H F - 9 2 * B K 7 * [ \ u u { o L : @ * 8 6 # * 1 ) 8 4 " ( " 4 ! C L W t g f = T B H 7 N ' " , 1 3 5 ) 5 5 # ' 0
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# $ " , , ? ! * 1 7 $ + " ! ! / ' " $ * " % ( ! 1 # 8 8 + ' ' # ' - 4 0 . ; L l h k ^ K K ? " G C ! / - . 2 * 0 ' % * " 4 ) 4 , - C V ` [ k t o D A 9 4 3 / & F , ( @ + 4 : ? : + I > C ) > = - 8 " ( ! % # 6 ) , 6 + ' + " & . ' 2 9 + 1 $ ,
$ 0 ( " " + * & ( ( " / , / . 5 $ & , $ + # " ( $ , " * 0 - & ! % % 2 & " ( 1 * # + 2
0 1 + " . 3 # / 9 < F Z d i T A D . 2 ) : % ( ! - " ) ! # : 3 & . * . ' # $ # - , < 0 , H \ e s V L M F > B & L A = * 2 ! ) ' ( 2 % 5 2 > G W a } h g d _ F * ' @ 4 ^ M ^ K H m w a q A M L D 6 2 7 7 , 1 / 2 A 7 $ - . > 0 0 _ 6 T m z b ] I . 7 N . - J : G R X ~ s y v o U : = H 5 & # - ? 5 + 4 ' ) 2 8 4 : # * , $ / 9 & < B ) D S a y P M 6 ? J + , . * - ) & 0 C - 4 - ! % . # & ( + & 8 ' 2 . 3 7 - $ ' 6 ( ) ' # ' < ; Z \ q m e \ L 7 C 7 F ? A P Y j { \ P ? F 3 < & / ? 6 J D Q j x l U ; 4 @ : 4 3 : * " $ $ 1 $ ' ! ) 1 ! # # - ! $ , 0 . + $ 9 4 . * ( & $ @ 7 = 5 2 ( ) - ! G ! . 8 ( " 2 & ' " / 5 ' + 6 5 + " & $ ) 7 . / . ? # + ! + 0 0 7 : ] = a [ } e y b d 3 S ; / A D * ( & 7 8 & - - * % / / O Q b r Y O W R H < X : A 1 T A Q x x g g 6 8 c : # 1 # ( ? . , 4 " = F . 2 + + ( ; ( # A 2 6 > @ ; H [ g n p e _ j F ` \ , U J I I @ W J 7 Z D E j L c o u g d u z ^ G F H 9 * 4 - * $ . ( & & 7 , 1 + / $ * % ) K [ _ n n t Y V ] @ G B > F 1 $ 3 %
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" ( 0 # / 3 ' ( " 7 % ) ( " ' + ) + 5 " % * * 9 ' * 6 $ , ' 2 8 1 , * " . G 0 / = 9 ` L e u z x x i R T U S A = E B 4 + $ * & 4 # % . 1 0 B E . L _ w x h t } y p | M W H H 6 ( ' 8 7 / & # / $ ) > 7 J : K V g v z b X b U G 6 . 9 4 ! @ $ * . $ - 2 0 9 + % ! + - ( $ - 5 E [ ] P ~ | ^ \ L > B 1 ; $ - 3 ( " * = # , & : 7 , ! ! . 3 ! 3 $ % 1 > . & % + * # ' E % ) ;
" & ' - ' ! " , - ' % $ ! 0 . - * # " + ' * $ * % 0 + ( ( + . * $ 7 " / 5 & # 1 ! * + # / , % 2 " 2 1 < > v H E U R R b w n e Z g U ? V v } m { t o h S G E ; G ( & < B 5 + + ; M F / 3 9 < ) 6 / 6 ) 6 K : b w x _ b O 9 E @ : : C D 5 ) ; F : 6 @ / % ' / 2 ( ) 7 & % * % 1 4 ) * # . : E J T ^ ~ b g j J \ D $ 3 ) A + * . & " - % % / + # 6 $ + " # " 0 B # . 4 , $ # & " 2 ( $ % & 7 , " + - # * A 3 ! ( $ . ! , / " + $ ! ! , $ ) F * H N c j q ~ o | } z } e E Q N % " . - 5 > * 3 & / F " 5 , # > 7 ( . 3 # - 5 : 1 $ 5 0 B 9 > ; - / ' ; 0 ) 1 , ' & 5 - + ( 4 * $ > ) 7 3 B I ? B T \ i h v \ \ ^ S o ` ^ } d a C [ D J : K 6 + 0 / / $ * 1 $ " - + ( 7 4 P = B n k \ v g r w ~ d X 8 D ; O # 5 " 8 & $ $ + ! 5 ( 0 # 2 " / . ) 6 5 = W \ d a h [ \ 9 < . 0 # / 1 3 ) 3 ' # & ( ' ( $ * , 9 3 , & 1 , - 2 , > , 3 " ? ) R K S [ u n b c U I I + " - # 5 G & 2 , ) # & !
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* ! # ) 2 ' $ , ( 4 . 4 3 6 I Q O w \ p s y _ U I a L Z ; E 0 / ( " 2 9 : / " + " - " ( 0 8 G ,
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* $ + $ , 0 ! 0 A = * ? Y I Z S ~ { j j p X q ^ Q f e l { | ^ k i f [ I C R J F @ E U ? ] > Z W { a l ~ } x | ~ W ^ ] W c g Y _ } y y s ] b N T X N 2 ! $ & ' 0 - 2 5 + % + 7 , " " + * ) = " ' 0 / " , 5 * < > . 0 ( ) / / $ " " + # . & , % 9 $ , 2 + * 7 ) ' % , ; > + - 8 ' 9 C ' ) " ( $ B % H 8 P g ] o } l } o m ] ~ l k p s q o l X D N ; / ? - ( 0 5 ' " 1 ( + G H M U c Z h z ~ f b g S A J 0 < ? 9 . 2 & * / $ E 1 @ + ; D . 1 E J T c ~ k j L N > 9 9 3 B R ? R > U N d r { z q f i T m Y X Y _ X G T H " 7 < N 8 ' 9 ! < 5 F P N ` g r g w t | y f r q \ o M ` 7 L C 7 C 1 ) > ; ] E 7 = U ? 4 X ^ d W r v f g o d L = 9 B S < 4 H @ 2 = @ 6 9 4 L M 2 A - ) 6 - 1 % ) # ) + % - ! ! 5 ' ' 8 2 ( C ' + I E H 1 I W W Y > = O ? Y Q 4 Q E > M M T 7 5 B K ? 3 C = / J 3 6 I 6 ) " 2 " 6 : @ ? "
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&&&222>==HHHSSS]^dggpqqz{{ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 173bd9877030494daa7abb3b8432d97eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-19_11-34_0525 3130Seq 12245 12245_Costion_K1356_matK_R jmadera 96-Well
K1356_G1_RKB_3730_POP7_BDTv3.mobSeqPop7z Aim@ 5PTC* +S
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r N a v g | ACAGAGTAAAAAAAAATATTAGTGAATTAATCTAATATAATTAAGAGCGATTACCTCCTTATACGCGCCGGCCCCTGGACCCCATAAAAGGCGCAAAATCCGGCGAACCGCGCCGCACAAGGGCCCGCCGAAAGGCCTTCCTACGTTCCCATCAGGCCGTTGGTCCTGCCGAGGGCAGGATGGGAAGGAGGCGTGGGGCCTGCCTCAACACGCTACCGGCCGGGAGGGGTGTCCCCACTATTAGACCGCGTCGCAAGGAAGGTTTCCCCCCGCGGGTTCATCGGTCACCTTACTTGTCGTGGAGAGGGATGGAACACTAAGTCATCACAGACCGCCGGGGTAGGAGCCTTTGGTGATGGGGACGCCCCCGACAGGGGACGACGTATTTATCCCTTTAAGGCATCAGCAGGAGCTGCCCTCGCGAAGCATTCAGGGAGTCCCCTTGCTTCTCTCTTTCAGGGCGGACGGGATCCTTGATTGACCTTCGGACCACCGTACGGGCCTCCACAGCCCCCCCCGCCACTTCATCGAGCGCCCCTTCATACCGACATCGTCCAACCAGGCACATGCATCTCTATGGTTATCCGTTACTGGATTAGTTGACTTCGTGGTTTGAGGAGAAATACTCATCCTCATATTCGTGCGTAGTATGGACGGACGACCATTCGCAGAGACTTCGGGATCTAAACGGTCGTATAGGGATTTAACTCGGAGGTATTATGGCTCTACTTCTACTACTATTCTCGAGGAGGGAGACTCATTATTTGCGCCGAGGAGACTCATCGTTTCTACAATCGAGGGAGACGAGCAACGACCCCTCGATAAATATCCTATGATGTAATTGTATATCACACATATTAAATTTATGTAGGTACGTCTTCTCTCCCTGAAGTTATAGTGTGTGTAGAGATGCATTTTAATGACCATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-19 13:05:13 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt . ASPt . B1Pt - B1Pt - BCTS / BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ,Y X DATA
,Y X =u DATA ,Y X ' DATA ,Y X DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS G P1AM @ l P1RL @ d P1WD @ sF P2AM @ P2BA P2RL @ z PBAS Kf PBAS G PCON a& PCON ] PDMF PDMF G PLOC @ VF PLOC @ O PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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& Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL SPAC AUv SPAC G SPAC AUv SVER 4.0 SVER $ SVER , , Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH G phDY big phQL c phTR phTR % This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt . ASPt . B1Pt - B1Pt - BCTS / BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ,Y X DATA
,Y X =u DATA ,Y X ' DATA ,Y X DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc y FWO_ GATC Feat / / J GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS G P1AM @ l P1RL @ d P1WD @ sF P2AM @ P2BA P2RL @ z PBAS Kf PBAS G PCON a& PCON ] PDMF PDMF G PLOC @ VF PLOC @ O PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT "- RUNT
RUNT 7; RUNT
& Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL SPAC AUv SPAC G SPAC AUv SVER 4.0 SVER $ SVER , , Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH G phDY big phQL c phTR phTR TFo@L15FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(j#1_!$y'+KbvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!#%`qpi)mazy,899UEU}k(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+V.BO(hwZ}JK2]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0