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d ,,,III^^^{{{&&&/99DDDNNNaaavvv ***333>>>GRRGRRRRRRRRGRRGRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR B!.2444433210/.-+*(%%! ] ] ] S S S v v v """..699CCDNONYYXbbbllkvuu}}} ! ! ! " " " # # # # $ $ $ % % % & & & & ' ' ' ( ( ( ( ) ) ) * * * * + + + + , , , , - - - - . . . . / / / / 0 0 0 0 0 1 1 1 1 2 2 2 2 2 3 3 3 3 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 : : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4120b8d061aa4814a5be61c7daa127faSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-19_09-47_0524 3130Seq 12244 12244_Costion_K1356_matK_F jmadera 96-Well K1356_F4KB_3730_POP7_BDTv3.mobSeqPop7z
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K eQ K!5 3$U GAAAATTTATTTTTTTTAAAAAATATCTCACACATGATCAATATGTGATATAAATAATTACTTCACTTTTTTTGGGCAAAAGCCAACCTAAAACGAATTCCTTTAGTAAAGGGGGGAGAAAGGGTCTCCCCTTAAACGATTAAAGACGCTCTCTGCGAAGGATACTCAACTGTGAATGACACTTCTCCGCCCGGATTGAGGTCTGCCCCTTAAACGAGAACAAAGATGGAACTACATCCAAAATCCGTCACCGGGCAAGGGTAGAGGAATGGCGGGGTTAAGGTACTCCACTCGCGGATTCCATCTTGATAATCAACACTGACTGAAGCAACGATATAAGGGTGATTTGGCCTCTGTCCAAATATTGTTCGGAAATCGGAATCACCTTCCTGGGGTTGCTCAGCCAATTATCGTGGAGTCCGGTGGCCCCCTCCCGCAGTACGTTGGTTTGCTATGTAATTTTATTCTTCTTCTAATGTTATTTCTCCCTCCTGATGGTTGGGTGGAGAGATCCCTCCCCCAGCCACGGCAAACCCCACTCTAGCATGGAATCTGTACACTGAGGGAAAGACTGGACACGAAAATGCCGGTACAACCCGGCCATCGCGAACGAGACTCTAAGTAGCAGCCCTTCCTACTTTTGTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-19 11:34:28 -04:00AEPt 3 AEPt 3 APFN V APXV 2 APrN ȏ APrV 1 APrX } } Ș ARTN ASPF ASPt s ASPt s B1Pt r B1Pt r BCTS BufT d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 0 DATA DATA DATA ,T X _ DATA
,T X E DATA ,T X DATA ,T X W DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! e LNTD 2 LsrP a MCHN MODF Ȇ MODL 3730 NAVG NLNE ` NOIS O P1AM
hl P1RL
cb P1WD
mv P2AM
w P2BA | P2RL
r PBAS Q PBAS O: PCON ` PCON ^X PDMF ? PDMF O PLOC
YN PLOC
TD PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT !; RUNT
7 RUNT " Rate RunN " " S/N% N SCAN 3 SMED
SMLt SMPL 6 SPAC AU SPAC O SPAC AU SVER 4.0 SVER SVER , , Ǧ Scal @ Scan 3 TUBE F4 Tmpr < User phAR phCH O5 phDY big phQL c phTR phTR &f This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN V APXV 2 APrN ȏ APrV 1 APrX } } Ș ARTN ASPF ASPt s ASPt s B1Pt r B1Pt r BCTS BufT d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 0 DATA DATA DATA ,T X _ DATA
,T X E DATA ,T X DATA ,T X W DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! e LNTD 2 LsrP a MCHN MODF Ȇ MODL 3730 NAVG NLNE ` NOIS O P1AM
hl P1RL
cb P1WD
mv P2AM
w P2BA | P2RL
r PBAS Q PBAS O: PCON ` PCON ^X PDMF ? PDMF O PLOC
YN PLOC
TD PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT !; RUNT
7 RUNT " Rate RunN " " S/N% N SCAN 3 SMED
SMLt SMPL 6 SPAC AU SPAC O SPAC AU SVER 4.0 SVER SVER , , Ǧ Scal @ Scan 3 TUBE F4 Tmpr < User phAR phCH O5 phDY big phQL c phTR phTR -Dq.$+WH x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&reA z3]fgif$z$AM=/:[q:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]gg+J4ow5A|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#xg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhIgtuu.m_(2hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;= AEPt 3 AEPt 3 APFN V APXV 2 APrN ȏ APrV 1 APrX } } Ș ARTN ASPF ASPt s ASPt s AUDT ` ` B1Pt r B1Pt r BCTS BufT d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 0 DATA DATA DATA ,T X _ DATA
,T X E DATA ,T X DATA ,T X W DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! e LNTD 2 LsrP a MCHN MODF Ȇ MODL 3730 NAVG NLNE ` NOIS O P1AM
hl P1RL
cb P1WD
mv P2AM
w P2BA | P2RL
r PBAS Q PBAS O: PCON ` PCON ^X PDMF ? PDMF O PLOC
YN PLOC
TD PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT !; RUNT
7 RUNT " Rate RunN " " S/N% N SCAN 3 SMED
SMLt SMPL 6 SPAC AU SPAC O SPAC AU SVER 4.0 SVER SVER , , Ǧ Scal @ Scan 3 TUBE F4 Tmpr < User phAR phCH O5 phDY big phQL c phTR phTR