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(%                             & # )                        d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR4AKPRSSTTTSRRRQPPNMLLJIHGEDBA@>>=;:8KKLDDD___uuu   (''000777>>>EEELLLSSRYYY`__fffllkrruxw|}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b9137a6bfd994f2cae101392663d879bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-09-04_11-32_02693130Seq1298212982_Steier_PueblaGap4-5_matkRjmadera96-WellK1358.D7_matkRKB_3730_POP7_BDTv3.mobSeqPop7zP7[}:Ws @x+WT%] / d | f P ? 4 3 ; L b w s W 6 pd\XUNA(](wT7U{kT4 {`C.&+5;3pZOIA3 !-9GYp|^8 w`L:) &?Xo8O^_R8j?<_%Kaf`TG=1!{E ),*%  ][{M^N{!~8r6 3hS~,Y<gRHFJQZera_t=mtS )32dV)P1{ih\B4/0366669=BHNVcv6HOH2lO9*!%.9FWluf`biqx}2z'c<?e"yspt:VhqrmcS< m^RG;.! %:LWXN<& (:KYbd_SA-      )6EXlhK/    !=]|z\<   '1=HRY[ZUMD:/#    $;Wv}V2 "+3771'  !*16<CMZiu|{sfXMGGLSY]^]^_cgihc]XUTUTQLGCBCED?7/*+08@DB<50/1452*     !! 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"$%$" #$"   !$%"    !#"      $/8?@<3' #)+("              $&%"  "&(&!   &,1453-$             "&&$    (,.,'"&'$ %))'$ !'-10+" !&(($   '.0-% "+/-'  (042," "''# ".573*  #%%"#%#      "    %+-*#   $&%!   %&$  !)/1/*#   %*+)$  !$%# #&(&#       "          !%&# !$$      "#!  !$$!    #$"                                                                                                                                                                                      kjv@iA7ALACKB.bcpKB_3730_POP7_BDTv3.mobtermGACGAGGATTAATACTCATCCACGAATGAATTGTTCGTATATTAGTGACTATTCTTTGTGACTGTTTGAAAAATATTTTTCAATGTGTACAGAGAGATTTTCCTCAAACAATTGACGATGAGATTGATAATGTTGAAAGACCGCGAATAAGATAATGCATGCTTGATCGTAAATTTGATAGGTGTGTTAATCTGGAATGTACAAGGGTGGGAGTTGGGAAAGTGGAATGAAGAGCGTCACGAGGAAAGTGGAAACAGAGGGGTGCAATCTACTCCACAGGATCCTTTCTTCCAACCAAAAGATAGAGATATCAACAATCAAGCTCATAAGCTTCCGATGGATGGCAACCTTAGGCATATCATCCTCATCATGGTCCTTATCATTCATATCGTGCTTTATGGCCACCGTAGGGTCATTATTGTAACGAAAGGTAGACTGATGACACGGGGAGAGGGGGGGAGCAAGTATCTCATGCGATCATTTCAGGTGAGAGAAAGGATTGACGAGGATTAATACTCATCCACGAATGAATTGTTCGTATATTAGTGACTATTCTTTGTGACTGTTTGAAAAATATTTTTCAATGTGTACAGAGAGATTTTCCTCAAACAATTGACGATGAGATTGATAATGTTGAAAGACCGCGAATAAGATAATGCATGCTTGATCGTAAATTTGATAGGTGTGTTAATCTGGAATGTACAAGGGTGGGAGTTGGGAAAGTGGAATGAAGAGCGTCACGAGGAAAGTGGAAACAGAGGGGTGCAATCTACTCCACAGGATCCTTTCTTCCAACCAAAAGATAGAGATATCAACAATCAAGCTCATAAGCTTCCGATGGATGGCAACCTTAGGCATATCATCCTCATCATGGTCCTTATCATTCATATCGTGCTTTATGGCCACCGTAGGGTCATTATTGTAACGAAAGGTAGACTGATGACACGGGGAGAGGGGGGGAGCAAGTATCTCATGCGATCATTTCAGGTGAGAGAAAGGATT&2CQ_ix$7DR^iu #.<GWaq*9NZg| (5CNYap}$1>JVboy /:GP^jw~)7CP]iv!2>NZeq    * ; F L Z j v   ' 2 ? 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N Y d q }    ( 3 @ M Y d q }   # 2 = G T a l x   ) 5 B N \ f s )4@LXdoy *6@MZer} (1>JUco} $-<HQ^kv'8DN\hr)3?MV_p} #0<IS`m{$/;JScoz",4CS`lw!,6EUet*9AM             +" "+)"""  ""         +            "# "+"  + %  "&$$ )20 +)+(- "" $!' +"+"" '(2.#$"       '"($ '""'"             $1$    "     2'                 +" "+)"""  ""         +            "# "+"  + %  "&$$ )20 +)+(- "" $!' +"+"" '(2.#$"       '"($ '""'"             $1$    "     2'    %L T="8-\M@ @F^U+R$d7= 7*Z UXUA  (X*4W Z2F2"KL),,&[6X1!=>KH /!G #YB,PCL`Y8&EK&IW(7"AS bE02`*bIDG8>O<M7.[;Q GX3>`@:6W]KR6b] 89-9!))/ (X]`T<FQ#]P^UMDW7BUb1W90@MBHK=3=8\CNYL` :@HFI?O _/<Y7\1JNNP?bVA,J350BO",CE7 G3>9b6*1]#% (?4>"@d;D2P FI4Ta7> O+VKA+U9/b_[Od%84+c2]L+7ZG\J6$];)(Q2<9a=_PB6L!]dB6<\3bKRC_(cR[HNcT/+-Y] /./c1(CV1M`"@.&?%*+6HX ][> \ RGA("(R?+$MDP9 XXL^ D?4* Zec?aZGF4|a:=0h>*F|w-ed Lz`I&zK$5opT*+YOmbv% ?bd #GZXf9e"\)i ft-8k>Q XN6AMLigD |9#,v;8VNH9u9FfuK*uo^#1hD>TL`;^M'3rCefF*R"u84k~B@RrY^[}d*s,nGr_1<R5_C7x&Ll*3s3D TJ~EC+U!2J-f PJ{xCfgEx<",w49?.BfTWhoY Hvu Hf z PYU|3 v;:4\cfCLa@5XciZ^ip?0~kZ .aQ:C?&>3-0  4$!50'>8p>e" &)P$@MGw{'(* vVshdr!mb$:Ubcr 1bM:7V=6s@`E85 AA &F@S;]:7^9,Q&(LD5^@8  CL" Bb+)>&/""oO" .(,s;,>!,Ia%B: !"O2@,6u \^X3]}8??R!6+Wr9E L "6<BV~zEj@{=. Y k7[R`M=!G-TBSz!t(HN~~ukilF aO-C=I)m''gLiJA $X?0p **?_8v.pp2P'vz2]tW#AC8<bs%kt(%B2sxMw{Sy5p\/"cKM$^cJl\24 >bIpKBoma,UuseTJp;NP$$08^x/xxVol&wM8#&zrYs!K>A G!R#Lh(g;0~Lc,F L88T op q>#mQrbM/=bpMn16;dr-Z@(9neKKJ!@3 /pHt!Q&\" p\  UNi ]46`"4-j)g}!U )%jn>hi./sr L1M(1<ah!D+3B=-GSpiP2W{4'faa&xcL%<Vo|?~Y1eIJ0CL?EgDt4)hIws9 ^(rB+gNj<Eih>RW*m/%P YU7;btgZy$|H(GQ~96CbC<\?i2]JaD<NSW ]kI&ob!gcZ  /[a7C"7jiVf.{M:AuR: iMF^I}($NC<NTqgakHf|.D$ACGTTTATAATTACTCACGTTCACCTCCTTACTGCGCCTATAATAAACAATACACCACCTCTAACCCCTTTTGGGGCAACTTGATCAAGACCTCGCCCCCAATATTCCACCCATGATGGCCATACCTATTGTCACCTTCTGACTTTTAGTTTACCAAAGCCAACCGCGTATCTCGAAGGGCCCTACGCTGAAGTCTCGTACATGTCCACACTACATTCGGGAGATGGGTGCCTCAACTCTCTACTGCCGAATGGAGACATCAATTTCCTGGAAAGGACACTAAGAAAGGATGGGGGTGCGTCCTCGAGGTAGGGATGAATGTTAGCCTTAAGCTTTTAACGCCAGCGAGGAGCCGCATCGCAGTCGGCGGGAAGAGACGGATGAGGGTCTTCCTCCATCATGAGGGCACTTTATTAGTAATATGATTTTACAGACTGCCCCCACTTCTTTTGCACAACAGAATTAGTCACACGAGCGCTTACCGGTCGAGTGCGCGTACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-09-04 13:04:17 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtsASPtsB1PtrB1PtrBCTSDBufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA '*NTDATA '*NT2DATA '*NTLDATA '*NTϠDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~C^LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM1P1RL-P1WD5P2AM=P2BAAiP2RL9PBAS $PBAS/PCON+PCON)PDMFPDMFPLOC&PLOC"PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT  7RUNT  0RUNT  6.RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLuSPACAl;SPAC SPACAl;SVER4.0SVER DSVER,,nScal@Scan3TUBEE9Tmpr<UserphARphCH*phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtsASPtsB1PtrB1PtrBCTSDBufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA '*NTDATA '*NT2DATA '*NTLDATA '*NTϠDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabSCFVocS2FWO_GATCFeat00SGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~C^LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM1P1RL-P1WD5P2AM=P2BAAiP2RL9PBAS $PBAS/PCON+PCON)PDMFPDMFPLOC&PLOC"PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT  7RUNT  0RUNT  6.RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLuSPACAl;SPAC SPACAl;SVER4.0SVER DSVER,,nScal@Scan3TUBEE9Tmpr<UserphARphCH*phDYbigphQLcphTRphTRA08^#5E^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]^>\JFb$&\c3bn={ZyB+V:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYXL-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bfkmb?9epAmw}5@yvY@m^K4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(+gbk1^>7(FTcSO[mOEW<#GnP Qc$@)k,g5?wAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtsASPtsAUDTllaVB1PtrB1PtrBCTSDBufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA '*NTDATA '*NT2DATA '*NTLDATA '*NTϠDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabSCFVocS2FWO_GATCFeat00SGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~C^LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM1P1RL-P1WD5P2AM=P2BAAiP2RL9PBAS $PBAS/PCON+PCON)PDMFPDMFPLOC&PLOC"PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT  7RUNT  0RUNT  6.RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLuSPACAl;SPAC SPACAl;SVER4.0SVER DSVER,,nScal@Scan3TUBEE9Tmpr<UserphARphCH*phDYbigphQLcphTRphTR