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"  !                           !**"-1(.14&*(%.1%/>/&               '++' !$!     +&#                    $                    &35        ($&#13'"&%   (%(& !!    # !&%                   '+ &%"-& )               #!                            %"&                                       #!           # #$                                                                                           ! !        %                              $%,+                                         d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR4AKPRSSTTTSRRRQPPNMLLJIHGEDBA@>>=;:8KKLDDD___uuu   (''000777>>>EEELLLSSRYYY`__fffllkrruxw|}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a2a5bfa9b10e41c0bae83c5b839b1056Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-09-04_11-32_02693130Seq1298212982_Steier_PueblaGap4-5_matkRjmadera96-WellK1358.B9_matkRKB_3730_POP7_BDTv3.mobSeqPop7z!#%(*-047;>BGMSZ`ejotz  zof^XTOJE>82-+*))))('''(()*,.15:=@AAA@???@ABBA@><;;<=>>=;::;<=>?@CIS`p/?JNNH=/ sjeceilnnlifddddca^\YWTROLJGEB>:61-)%!  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"(0:DMSUSLC90*).8FVdosqhZJ9+   -9DMRSQJA7,"     !$&),159=AEJPW^foy+AWlzeN6 "-8CNYdnw~~th\OB7," {qg_XQJC<72/-+)(%# !$&()+/4<EQ]jw~vme\UOMMPUZ_ceec`[VQMHECCDEFGGEB>:630.--.27=CHJIGD?:4,"  !&,3:AIPW]`ba^YURPQTX[]^\XRKD<5-& "(/6>FMRVVTOIA92*$!"###"!#$%%%$$$$%%&&&&&&&%$"    !$'('%"!'1>LZfosqj_QA2% !#$$#           #'+/369<@DINRUXYZ[]^__^\YUQLF@:50+(%"          "##" !""       u{"@ѿHAAMAgKB.bcpKB_3730_POP7_BDTv3.mobtermCGGGACTGTCGTACTGTGTCCGCGCATGATTGATCGCTATATTTCGTGTCGGCTTCAGCATTGCCCGAAAAGGAGGGCGAATCGGATGAATCAGCCCAGGCGGCTTATTAATGAAGAGGTCCTAATGGGTTACAAAATTTCGCTTTAGACAATGAATCAATCATTGGAAAATTGTAACTCTTGCCTCGAGCTTCGTCATAGCATTATCAAGTGGGATGAGTTTCGAGCATTTGAGCCGCCCACTGAGAAAAGATTAGTACTCTTAAAAAATAACCCACAAAGTCAAGAGACTGCTCGGGACTGTCGTACTGTGTCCGCGCATGATTGATCGCTATATTTCGTGTCGGCTTCAGCATTGCCCGAAAAGGAGGGCGAATCGGATGAATCAGCCCAGGCGGCTTATTAATGAAGAGGTCCTAATGGGTTACAAAATTTCGCTTTAGACAATGAATCAATCATTGGAAAATTGTAACTCTTGCCTCGAGCTTCGTCATAGCATTATCAAGTGGGATGAGTTTCGAGCATTTGAGCCGCCCACTGAGAAAAGATTAGTACTCTTAAAAAATAACCCACAAAGTCAAGAGACTGCT7<JSZcz -:N\hs)4ALZgpu3Qcx'18?HSbnx &3@KWk|'2;DP]hs !-:GR`oy %2<HScmz    ( 6 @ M \ i t    . 9 F P \ e v    * 8 I [ g t   - < L Z h s    + 5 B P \ f r  &4>JXeoy7<JSZcz -:N\hs)4ALZgpu3Qcx'18?HSbnx &3@KWk|'2;DP]hs !-:GR`oy %2<HScmz    ( 6 @ M \ i t    . 9 F P \ e v    * 8 I [ g t   - < L Z h s    + 5 B P \ f r  &4>JXeoy                 2    "223 #"$' ($&                           "                     2    "223 #"$' ($&                           "    *6K`COOTR9AZ %^"]aa,WW'bdOK5 ] %&%_WOPbU0H @&Y6KJ=)=Q"A_?SXQ6Xbc*9=( 9.P54,#C0 JD_,J^3?61&=<J=*HBB0*B% #>O+@:90B9> K[*&4 6! *]9)KT/ 9!V:F"B>Y#W3FM^_!OW7K^D"UbQ @(HJ]>_ 3HQ,1C0Q? b@]:3/9c ,/Y;L4%=DKKVO?IFaU`ON0M3-_!%Rzg0XH y  >%,leJOW ]- *ad Y Y9li?>O  0iu 5SVE~29_Qp WAa O&~6I=g RuI $>Kj}QSQR dODt&`,jA4mHpN@%B_X k$9;|n2,TbZara|)qzvN6i!LSEw` -.K &+/|Rvj|} eHs3}9^@ 86GW~S?v"D%6,c9 dFN{@8ZcC3,D' >qhx28Qp 2L"&eO~vDI%& V' B C*7 IS)93 /vXXap= 8W"YRBJp`CT29},z H "dRhJ5pfi _Qi<>3Xjr yU&EB kU8 pb=s9pjdk%+MR/56Gm&T H;Uz)Mz.O@UG= f|naNu-j+DD1DxO=vpe .: 7|U7I-WA)Mk8 4t 6!_{ LGZN2{_L UTXdTf   pVh Xo` H2b]Tg-{: h>us!7'F $I5?PNR}& bqhl.StpCk 2Nf0`&GCAACTCTCTCATTAAAAAAAAGCACCCCACTTCGCGCTATAAAAAAAAGCCGAAACAATAGCGTTCTTTGAAGTCCGCGCATCCGAAGTAACCGGGCCCGCCGGCCCCCTAAGGAGTAGGTCCTAAAAGGCACCTCAAATCTGCAGAGGGGGCGGGACCCGCCGATGTCTCGAATGAAAGGCTTCTTGAGACGCAAGAGCACGGGAAGCTCATATGCGTGGGGCGCAGAGGAGTTTTAGACGCAGCGCGTTGACTTGCTATCCGGTCGGACCTGTCACTGAGTGCAGAGAGATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-09-04 13:04:01 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtcASPtcB1PtbB1PtbBCTS2BufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &MnDATA &Mn2DATA &MnDATA &MnDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~0|LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISdP1AM'N&P1RL'N#P1WD'N(kP2AM'N-P2BA''/UP2RL'N*PBAS''PBAS''PCON''"PCON''!PDMFPDMF{PLOC'N3PLOC'NPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT  7RUNT  0RUNT  6.RUNT  Rate RunN""S/N%\SCAN3SMED SMLtSMPLuSPACAn8SPACtSPACAn8SVER4.0SVER 1SVER,,nScal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtcASPtcB1PtbB1PtbBCTS2BufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &MnDATA &Mn2DATA &MnDATA &MnDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab@_FVoc@NFWO_GATCFeat..@ GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~0|LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISdP1AM'N&P1RL'N#P1WD'N(kP2AM'N-P2BA''/UP2RL'N*PBAS''PBAS''PCON''"PCON''!PDMFPDMF{PLOC'N3PLOC'NPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT  7RUNT  0RUNT  6.RUNT  Rate RunN""S/N%\SCAN3SMED SMLtSMPLuSPACAn8SPACtSPACAn8SVER4.0SVER 1SVER,,nScal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTRk&zt Mi3rs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!PkWHt/rqi1jc:FJ3),~.|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_z x:H-p@~KCozPk&OE6OLZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#