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"                                                                                                                                       d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5V`deffffeeeedcca``_]\[ZXWUTRPOMLJIGMMMEEEaaawxw   %%%---555===EDDKKKSRRYYY```fffmlprrxyx~~~22223333334444445555566666777778888999999:::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c6f9c0a503bf4dce8249dc6603f297c1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-31_13-30_02643130Seq1298112981_Steier_PueblaGap4-5_matkFjmadera96-WellK1319.F4_matkFKB_3730_POP7_BDTv3.mobSeqPop7z?y*5Kr"[4r'] th - ~ A G ) _  LAnS+l   8 a 7nXNr^Yao}wwr=}<T(hD"~tnpv,V @&z 4=AB>1z7v<yqjccmO [R,0)|!4 ;x.i'g{iaafovwqbK.lN1sS8&"2Km8v#Kcmi[F-  _xV:$ !)06;>AFLU_hnlcUE;<Oxs )e`<InYRQRQMG@81-+*,.29CPat~uj[I6# 7nT8TO)s- 9(kI6./3:?CFFC=739P} WKqxbk7?2~T6"$4G\p~oYA/(1Ks)m 5qV`IdQ/PtfG, *0/' 0P{:dWg4   2Kbt|zlX@( %.5994) Drj]]wNT2 Ez ai$@I^;& $>\wlP6!#2BPZ]XL;'Aqk#iD=GSXQC89P2Y\Q,:FKIA3$   *RY:$!&*.5>FMQQOLKNT]iwn_XTQNIDACIS_lw~qjqK?T$    #0=EGC8+(-+# D"qIS" (8GQUSME=6/%$SfS'*Zkl^$  2FXbaWD/ %6DKKC6'    #'&    [BS@*M ,C]utg^ZXVPE6% !0=FIG?5( :ugD4K    );Pg~oP1"F}Zo2W) 1AKONOYt+gn3^0 #?x8 CHC~V[ DaaE^//RvdE/$(8Pi}v_E.&.32+!    $CnJ !!  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"'31'+1""(31"'.+332+8;>;;;"++.##"+)).2("2+;'332(("11313.21+1(""+111+31" "+(012#8(22+';;1"" +&2+'3+23+  "  "   +1)11+     '       '"   % "                          2,EM@aYO5(6/QW+],` #?) !#:>J& ab2:"+R[R WDAb!$\016/ MDG?>F]5C T.@1,$cT;A,Cd.[?2D^Gb;)WE `.T=2;=*]UEH,]9F'4<96H_0;QE  ,JRB>#M #$( /0A" 6d"A8'CAX( ]]M;B A CN(_@$K 75+c6 FW#@/dW)XM# 296B/PYU8$#C`[+1F7+"J-&R :I;&[^L!9+ OX'L?]L=1TV/*8EL G*'Q M`3(;/'*I/6' 1H0]8DW+F*,'`5]>".?AE2&RF<.ccR`aJ^F2$W4('J.C0^Ac&2#]MO4WF 20`'L6FM#aZQA`/8B82-";)@5/)J[ O 99PUZoBjX Xr_\DA <H7a#v m }c: 8a@&p:6YeBIU0) U+h.ld# SGQC6fwRT93+lg;+mm, ksXbFgnS\OC4L X;_fjdNP0aX/Qu^ 1/-HmS`3Re)r^Ensns[ OAZO=$pwJLk:06 Uq,P:7ZO^.M0iYdTKw2W@m2=(# tEn5_ E> IK$ KhP/ pCy{}ak5Gm :~'# M"Hp Vg#H{Fd122j{icq^H{d@@?A8N O>!c @A^Q(3F,A%$7424   #"! % lM$0   !'h'#  Kp&#YU*     ! $&;M8#QKONKX:v!_    Nf#g -/`?B>5  u"'+$G@p i!3hOOoJ2! q . )Q*  n :8;SX*9<U' d)wp,/X*KW &"J?LK( t!l%8vu]8HOt\g{)^%.pY QR$"@50:FTlp#/AgZ66Y)phZYF}"4XXG mx}4e]pg5$Tc]dd~\F82 `Rx!c~u05Ix!+U# [qD?o&,8 a9/ MpV/Zpzdv!qEm& [ko   k@3sjNk^Tq)Hgsbk1.5cX'K*SmFzC|"#c).~OInY4Z?wNAm Glk]&Z.dre&!jQbe_b@GORdkB;E~;~F-|N@wskI/Xbm*%j5c|6kLy; %oqsc3Ly@qJ`1YqdzDdx9RI X>C#p_gk#"Hnb^D)} Rep+K`GwQk%Y ieJPc:"}C~ $c)[cI+s[ pzz1bGvIwU Gh X)sGGGGATATATACAGCGAAGGAGGACCAATCCTACAACTGCTTAATAAATCGTCTCGACTAAAAGAGCCCCGGAATTGTGCTCTGACCCTTGCCAGGTGAAAAACCCAGGGCCATCAGGAGCAAAAATTTCCTGGGCATATTCTCGGGTTCCAGCATTGGGCTAAGGTTAGGAAGGGGGGAAAAACCCATTGTTGGGCCAAAGGGGCAAGGTGGACGGCAAAGACAGGCCGAGGTAAGGATACGGTCGGAAAAAGTATTACGTTAAACGAGTGAAAAAGGGCGGAGCAGCGGGGAGAACGTATGGAAGATTGGGGTTATGAGGACGTGACCGAAGAGTGCCGACGGAGAAGTGACGGAGCCGTGAGTGGGTTTGACGATAAGTGGGTTGGTGCGGGAGAGAGGAGAGGTGCTGTGGACATCCGTATTGGACGGACTCGGGTCTCGAGATGGATAACCGAAAGGCTCAACCCCTCCGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-31 15:10:14 -04:00AEPt3AEPt3APFN oAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtB1PtB1PtBCTS4BufT ACTID CTNM CTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA fDATA nDATA vDATA $IxDATA $I2BDATA $I| DATA $IDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ĪEVNT ĶEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~3/LIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISP1AM"sP1RLP1WD&+P2AM-P2BA1SP2RL)PBASPBASPCONPCONPDMFXPDMFPLOCKPLOCPSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 'RUNT RUNT )Rate RunN""S/N%SCAN3SMED SMLtSMPLISPACA}SPACSPACA}SVER4.0SVER 4SVER,,~Scal@Scan3TUBEB11Tmpr<UserphARphCHphDYbigphQLcphTRphTRFThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN oAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtB1PtB1PtBCTS4BufT ACTID CTNM CTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA fDATA nDATA vDATA $IxDATA $I2BDATA $I| DATA $IDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ĪEVNT ĶEVNTEVNTFTabCFVocCFWO_GATCFeat..BGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~3/LIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISP1AM"sP1RLP1WD&+P2AM-P2BA1SP2RL)PBASPBASPCONPCONPDMFXPDMFPLOCKPLOCPSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 'RUNT RUNT )Rate RunN""S/N%SCAN3SMED SMLtSMPLISPACA}SPACSPACA}SVER4.0SVER 4SVER,,~Scal@Scan3TUBEB11Tmpr<UserphARphCHphDYbigphQLcphTRphTR~.NbmnjW,DK >M7NJrj(R0e`EsaP=&&y:"0kfJAROX#Cc4l(Wf|Yu)+xm_'#k=sDa)x+7E2]^=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&4+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&]+;X$O6%nOp$eY\PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AVVu>\&_MBSmv^Y6UziPtJdQex-U[dPz;+hj3T'7_6t;:F8/GT&MsU~ik]^|uFA&v"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"BmWM@A<}1X*#PE|G#1Q$,#)+9GGApKAbZ]"Po1Cd}F<_`VWY+a:WkeC.%oh^5Q1V?|&@8je]i^mc+=I3WW' rD/gzoOf4v4*un,T(k"T'ogLOE2(/(W,XD3J6EX! w+[_3sh("tY-a$c}Wq=wLi|{y?hA(ue6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t