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"   "  "    " '  "!)(& "       '          !!#                   #                  d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5V`deffffeeeedcca``_]\[ZXWUTRPOMLJIGMMMEEEaaawxw   %%%---555===EDDKKKSRRYYY```fffmlprrxyx~~~22223333334444445555566666777778888999999:::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a5b49b699182489f947b8e7672d8d7beSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-31_13-30_02643130Seq1298112981_Steier_PueblaGap4-5_matkFjmadera96-WellK1313.A3_matkFKB_3730_POP7_BDTv3.mobSeqPop7z 3Jb|-KanzL{'F[hr}e7zcYVTJ6ypg`]_hv&9GPUUM=$oQ,qd[WZcs9eUoO3}nd^[XTQQSZbkqtvy})Nw4d`5 c9xsrsspi^RG@<<<;97310049?FLQW`p0Kh"nO,cPHGHHE@:532345553/)! &2AQctycM9(  '.220-)%!(7K`vgN;-$&4G\rnO/   #&'$! %.;Laxm[J;-!  ".>Qj}i\UTUZ_ekookd[SNMORSPI>2& "=_pM0 &@a@a{j;p:&:Obqb?" %2>EHIIHFA9/""ae#GNC9 ,Hk{cJ26Wzx`K:-#      0Mn}aH6)!  3J]gf[H2 /BTahg_SD5)!!9Sk|vlbYPH@9420.*$  &,//-)$&,0210-+(%" !$&(*+-/36998779<@DFHJMQV[]\ZWUVX[^`^ZQF9+ #@brO/ )4<@@:0#   O2p[u: '4>FIJKMS[bd_SA/   $#       $&'%!      !%''#     "#!  !&'%       !!!)-,'         #"                     "'+,+("                                                            ceiq<c/aO`|;@O L ' q 8 b } ~ j J  K b qS]!z;P"i>e=|nd^[YXXWWVVWZ]`a]SF7*!"%()))'$ )7H\r"FgzcM;-  (//&|jQ2wmhinsvussv.Ry#Ls!('m2a6"?`"Sz|iT?+  %(%"1:=<:862(d-eN=2,**+*))-6F^}!MvwwjH $\CZ]N1 o3oVG?:62003<IZqA_u~sdVI?71)!Bb}{W%yN1)5ANYbgjlns~L3UcX4`iE* 5[7cf@~si^PA3)$#$%%#  )4@LW`gknrx@nP|pjjq{~nW>* '0=QkjR@3-,/3798642248>DKQTVSNG?82.,++-07AM\kw}ukdfsQx=/ /Tu_N 8j0F_|`># !"  0H_pyyqcQ>,'1796.$ %1;BDC>941137=DLSXXTKB:9?NbxkU?* "7NcusbO;+ ,Ln|aH4&   &,1430,)()*,-..-+($"(-011/-+(#(>YtiJ-%?\xdF+     +D^wmR7   #$#      (fzTOIbsjC' &152)            #$!              ,>Q`hf[H2           !                                                                  0AP[^[O?,                                                    #,5>EKRYco|4Qm=b 3Z~2Qs4DP[ftygVE3c?X0 ~ske`^]]`eo}  :WwkVA*  ^8c<qbZVVX\akz&?Zv&1:>:,\:}tnkjklmmmmnooppprv~4QknS6m\NEA@BHSc{Gpc4p_WUYcp"AXa]K. eF/%/9AEFB:0&#0=HOSSOIB<768=DKRVY\`gsmVC4+)/@[:QUI.  ;e}Z(W7"3V/[wzfI(|k]SMLPXcnvzwpf`_fv<S_\K-&`~J]?( -;HPSQJ@5)  ?mHcgT.YW ILxD! 3S,dY2Bq{6k?$%E~Ji]cmAh,    2EXhsxuk]K:+         "#$#!     %*,-*%   "4I^p{}ugVICJ]{gYUW]bdb]VOLNVdw|gYRT]iv~ykV?( %('#*=Thv{tdM5 '1<EJLH?5+',:PkmQ8$    "(++'! '=UjwyrbM7%$9VtdB& 8\`B)   .BYqmS:'!/CYkw{vl]L;+ 'Jz%HP;U)         !##     $'&#  !!""!  #$"                                  !                                     $%#                    !"!                                                  "$%&''()*+,-/134689:;=>?@ABBA?=<;:;;<=>>???><963223220.,,,,-.--,+)'$       JLNRV[]^^_dlu~~xuu{9Tm;\|rY;vdQ=(yvwz #-<M^n{q^J5|unf]SLINYj} {z| ,6;<;81%obVKA7.'"  '3@Pbtq`O@4)! &-6BO]ku{|xqiaYQH=1%,Fh<njBtZIDHVlyk]N>0$$-7BMYfsvi\PD8-$$3Jk.ax`TVk,.fnJ0!$(-3:CN\jz}m[I7(")/3431-)#  %P!gG`A-!%+02444433237D_^2[0E(q!X0$+0442,&/Hg )=B8!bH5*#  ")2?Rj~Y9!6SphI.3RtkTA1%%6Javxlb\\ais{~uhXG5&   #<ZyfM7&    )@VivxrcO:)"'+/132/+% !!  &/6<AFLU^gmrtuvy}|vpke_XPH@92*$  #>_mH(&@a~\<!)HknN2  '-11-&(CbnM/  7Wx~cJ6*&'*,+&  !##!  +<KVZWL<*  %-1/)!         "$%# "%')+/2455557<DMV\]YQH@;888861+#          "&(('$                 $''%!                        "##"                                                  jo[@أA ADXA jKB.bcpKB_3730_POP7_BDTv3.mobtermCGGGATGGCTGTCATAGTTAGATCACTAATACCATATTATACTTCGACTTTCTTCGTGCGCCTACAGCCAGGAGATTCTTCATACTTCGACTTTCTTGTGAGGCGCGACGTGGTCTTCTAATTGGAGTGTTGATGCCGCTTCTCCCATCTATAATAATTGGACTAATGTTAACACTCCGTGAAATAGACTTTACAATTTTAAAAAGAAGATAAAAGGACTTTCGAGTCCTATATATTTATATATATCAGAATACGAATTTCTAGTATGTTGCCTTGTAAACCATCCTCTTTTATACGATTATGTCTTCTGGAGTCGTTCTGGAGAGATCCTTTTTATGTGGGGATGGACATTTGGCGGGATGGCTGTCATAGTTAGATCACTAATACCATATTATACTTCGACTTTCTTCGTGCGCCTACAGCCAGGAGATTCTTCATACTTCGACTTTCTTGTGAGGCGCGACGTGGTCTTCTAATTGGAGTGTTGATGCCGCTTCTCCCATCTATAATAATTGGACTAATGTTAACACTCCGTGAAATAGACTTTACAATTTTAAAAAGAAGATAAAAGGACTTTCGAGTCCTATATATTTATATATATCAGAATACGAATTTCTAGTATGTTGCCTTGTAAACCATCCTCTTTTATACGATTATGTCTTCTGGAGTCGTTCTGGAGAGATCCTTTTTATGTGGGGATGGACATTTGG#,7BLao $2AO[gs &4BNYdr#1<K^jx(5@NXcq} (6?O[ir~*7HUdp}#1;GQ^it%2<HV`m{    ) 5 @ K U a n }    & 1 = J Y c p    % = J T _ j z  ' 0 = E S ] h u  " . : E Q [ e r { "+8CTbr{!+;ERZdt*5ALXalx#,7BLao $2AO[gs &4BNYdr#1<K^jx(5@NXcq} (6?O[ir~*7HUdp}#1;GQ^it%2<HV`m{    ) 5 @ K U a n }    & 1 = J Y c p    % = J T _ j z  ' 0 = E S ] h u  " . : E Q [ e r { "+8CTbr{!+;ERZdt*5ALXalx      *   ")  %8!   ""'&                                            $                           *   ")  %8!   ""'&                                            $                     D 04TR$5a]%&?FPYTI6 B(7  <V^`79+HFS.LW=HN==GS8> 8\,L:7Z5S.?LY-1(D`1E%DCTI0TFZ\9Z67b^=*1$ 8TD+'AQ4cZM%6C#NdMS"2*T:C*]E\D@04),,&7:,0L##K ) -9#U _6 I*G'a.] ODYX)E9L*()9W/R89QOD*+`,&U+N#U#">Z SD)!7Ua+!M:_ a+I7JcUTT0+V<`7BbS*'Y&4KVSE#C'9/G$(+O)#87bK)1UUY4SMN;9,d]=+?!C/C! [a jCRs?|<9p9o~qU71r$Oc~H)(w' VnEHF|d x !8h D1*"FFrh6QJzK|OwG)J!U1f.m(B~1cfi?x}v@QAsO!n@@5|~s OT 2WQ36c@] 7#~4 )<)W@2:~nCKK]%z._oGXxk,zs|J[ Hh  `$0U-(>siZU#t2m/JEFXz [2sJ:J.\K@Z:`K6%,J5! -3K,#ynwJ(^lsf0gFz 0   |7w"= Jf.HL.}<4 %c03  7Cp\IpGdd8@$7 Vm75}&E}8jZivt[>ArVDF.uT1#sq48^#jsy-O8bdU8AHeSyKDAGG?SpF IY3s8FD\ypcFNr ~pf6#Z2nc3mAok:x~* d>WHg#ChXwV{yg a <(PQ&_I|mM~KRA~U#p >o <(C_}l=}:4O/{rczRqra0SL%)>CdIA;?>Cd$OHAiD"a9q < g7\sT2dFuaU*[KN/HRU11?F(Q\{ Pv3ab2+q@rGtT|DPa;)^;6yy7P{^9G_Yw^k4z\3:yb\sG*j8oFqZ~GAAATAATTGTGTTATACATATAATGGCTGGAACACGAGGGTCGAATAAGAAAGGCAAGAAAATATCGACTTTCCCCTGGGGGGGACTATTCGCAGAAAAGAAGTGCTGTCCTCTCATCTCAGCCCCCTAATGCAGTCTCATCGTAGAAAGGGCGGGGGTCGAATGCCACGTTTTGTAAAACTTCCTTTGAACATTCCGACTTGGAGCAGACCCTCACAAAGTTGTAAACGGGAGCGCTCCAGGGCATACGAGAAGTGGATGCTGGGCGGTAAACAGGGCTATGATCGCAGCTAGAAGGGTAAATAGTACTTACGCACTATCCAGTGAGACGGGATCAGAAAAGGAAGATCCGTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-31 15:09:00 -04:00AEPt3AEPt3APFN oAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtB1PtB1PtBCTSBufT ACTID CTNM CTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA fDATA nDATA vDATA #jFxDATA #jF/LDATA #jFv DATA #jFDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ĪEVNT ĶEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\LIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISP1AMcP1RLcP1WDcP2AMc3P2BAccP2RLcmPBASccfPBASccPCONcc PCONcc UPDMFXPDMFPLOCc PLOCcPSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 'RUNT RUNT )Rate RunN""S/N%SCAN3SMED SMLtSMPLISPACMSPACSPACMSVER4.0SVER SVER,,~Scal@Scan3TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN oAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtB1PtB1PtBCTSBufT ACTID CTNM CTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA fDATA nDATA vDATA #jFxDATA #jF/LDATA #jFv DATA #jFDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ĪEVNT ĶEVNTEVNTFTab.?FVoc..FWO_GATCFeat...GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\LIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISP1AMcP1RLcP1WDcP2AMc3P2BAccP2RLcmPBASccfPBASccPCONcc PCONcc UPDMFXPDMFPLOCc PLOCcPSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 'RUNT RUNT )Rate RunN""S/N%SCAN3SMED SMLtSMPLISPACMSPACSPACMSVER4.0SVER SVER,,~Scal@Scan3TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTRkl{Zq\-4/CeG1{?{sb: MOsh9VMR5t(jICx@VFmYK3AQZ`K'H:{GKipM`{`3e&Vc:z x:H-p9bgh`U*{Ga$FwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]Z`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ/^idT@CzS[~k8N'O?6pkC:J|bU,iPis|KRaD678w!lVcr,,z^qXnF){3&H9n9f}~E\\&(6Emc9tp] j MfaqQlYm.bBT9zH'zeE^O{U,\)lOY%}WeoVL6e-IZ(0\5?r,]!:!hBjKMCW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1@ -Tr+ TW>qO9`}!&RW8X&tG98PbC.Ju5%2-*"KjGf%_^XVXK,\n-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9ci|YZ(dNVE]&uSM7XNGgg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9U|.&xW$'%7PxI+5?-Y.pa6:!.tkj^4E2rW.X}=_;!;`sEY5_}4]'Z5P%?p MfaqQleA./q}.}:>JWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTcS[7}?e78SG9}_HtV2}2?Yk6]DZSPW~VW<77UAEPt3AEPt3APFN oAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtAUDTll