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"   ,  (*#  &        "!  %             ! !  ! # &# '!  $ (      *$5,7#0&.58-6&&&&-$ 9($%5+$1,  &         &    !("&"&!%>1'( $2 %))            "!!         $!"+* !)/!&96%*H@@(."&'5(%# -#/=(# ("                          !   %*  &  !""/ #  $ "       0    %+ "" !$!&!  #                # " # ! $&                              !   -" *#.             #! #  ,     ' ##     ! #-!$ %:, @<#",1" +%! -("&%&&'$',.35<?B98(4?:9IJ?R7>J8JZ4;?;P5;MBC73A'#53&#52-(*&*"1+ (%"<#" $ %  (     ) # *&  !                                                                                                                                                         d,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5S^cdddddccccaa_^^]\ZYXVUSQONMKIHFECLLLDEE`aawww   ###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2ba7fed656f343e1a2ab9d28c1536659Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-Well K1364.C4_psbAKB_3730_POP7_BDTv3.mobSeqPop7z,AS[T>!>UYF ^ *06;:1"2\aA9JmnNETrX. &Ih|~pW>+$)4<<4'*>Q\^\\crvQ 0D8~z||w<&U%.RoxlO0&:HI;" V#\(2?(GQF%U3RXhz__ta' ':EIHB90++17:5' 0Z,V:<Bg9 $5:3&)Gq.KZWC"tW7 /DIHPnS0]}LpL&t2"E_pz?q}wmdg %,809 lLz8 =Xghb`k)"+ Q V pX+_J:^Jg u X O^AxSB===>Fbi\+f[}e_j%.L Kx5H "$   $#  &% '05>QyvD@2wg`_adc[K7! 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"   *692%"%#    '.-# #04.# 0G\f_I, #**&   %6CG@3#/J_ieWH=750%1BF;( (02-# "4EORNHC>:4*  +1/' ?`y{fL::Lm~dL8,+5I^kkZ>&;GG;( 8XtufYPJJQd xswysmfXG709Rsl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cv@#A]AGA5?KB.bcpKB_3730_POP7_BDTv3.mobtermAGGGGGGGCCTTCTTGGAGACACCGATTCTTTGACGTGCTGCTGTTGAGTTTCCTCTATAAATGGATAAGACTTTGGTCTTAGTGTTCCGAGTTAAAAAAAAGTAAGGTCTTGCCTACCCTCTTGAGACCCCTTAAAAAGGTTATTGCTCCTTTGCTTTTTTTTTTTTTTTTTTTTTCATAAGTGTTTCTCCAATAATTCTGAGAGCCCCAAAAAGAAAGGATTTTAACCTCCTATCATAATTGGAAATTCCAAATTCTTTTCCAGGGTTCATCTCGCAGGGGGGGCCTTCTTGGAGACACCGATTCTTTGACGTGCTGCTGTTGAGTTTCCTCTATAAATGGATAAGACTTTGGTCTTAGTGTTCCGAGTTAAAAAAAAGTAAGGTCTTGCCTACCCTCTTGAGACCCCTTAAAAAGGTTATTGCTCCTTTGCTTTTTTTTTTTTTTTTTTTTTCATAAGTGTTTCTCCAATAATTCTGAGAGCCCCAAAAAGAAAGGATTTTAACCTCCTATCATAATTGGAAATTCCAAATTCTTTTCCAGGGTTCATCTCGC  /9GR`iu*:@JP[kp~ +:CIRZfx #/9FQ^ky (4CR_emt$2<JValx):DNWam{+5>LXcr{ !-;IPZiu}   + 6 E O \ j s }  ) 3 = J X d o     & 5 B L X f p {  & 3 A R _ l {    /9GR`iu*:@JP[kp~ +:CIRZfx #/9FQ^ky (4CR_emt$2<JValx):DNWam{+5>LXcr{ !-;IPZiu}   + 6 E O \ j s }  ) 3 = J X d o     & 5 B L X f p {  & 3 A R _ l {                         ""    "                                          ""    "                   YA1WI_2ZaDH? S;8*RPNO& H "?\Xb.6 ULD#4@?`<^[]B#B\^%TA8T ]#KB+> bW9c6V74^?O#K7XMZ> +!1*9="Y`#HT8`J-Z``aEdV (_8 K`Bc&RI[ZVW])[6 (b` :bS9O>Y$&b_ H60_HXP2/S__SFPZ F1 9__2-)_L2 D%2 \Q_=QYGM)L)=-]9c hQ .iu- . u{#c/JDW[}$unBw.1Dha' Y ~J 2>*=g-@ /  y%X`Yk:0Bw OzLjgRAq)duWn6d , S.H?lUm0gs 4xf`]+d%3qOe dPr/L0_'*18-P*'%WN q,I0!RDN?9dw,K q&1p\R,z: 2&B@J1agNDby|%Q@q]5aW/EdQh4,dLH$U7p+LBV.,________________________________________________________zJFttDppgl|Qq:pV KpSAdy+yq,9`dhm`{lqd4~u6NW4 ,J0{I',ed3"dB=)YVd-m}%s;J18dR0 x;"=Yvp.9*' h h T B hj U5i9i#&jav FO#85x(O#kS_2 Es8iTWdO56-<OCT8"5vj\<MH;Sj3M)*s o$7XXa0"^%v$nlxUWV]:1%~GC}b &GAATTCCCGTGCTCGCAGAGACAAATACTCCAACACGATGTGGTGCCCCGAATAATGTGTTTGTTTATGACAAAGAACACCCAGTCCAACCCCCTTTTGGGGCCCCCTGAAGTGTATTTTCTCCCCCCAATTCATGGTTAAGGGGCAAATAGGGAGAACCGAGGACCGGGCAACACCATGGGAAAGGCTCTACTATGGGTGCCATCAAATTTCGGACGTACGAAAGGCTTCTTCTATTATGACATGTTACTGTTTACTCCACGGTATAGGAGAGAAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:01 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS1BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &MDATA &M2ZDATA &MDATA &MDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~0_LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM,&P1RL,$mP1WD,(P2AM,-P2BA/IP2RL,*PBASPBASPCON#WPCON"APDMFPDMFPLOC, PLOC,PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLtSPACMSPACSPACMSVER4.0SVER 1SVER,,nScal@Scan3TUBEE1Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS1BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &MDATA &M2ZDATA &MDATA &MDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab@BFVoc@1FWO_GATCFeat..@GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~0_LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM,&P1RL,$mP1WD,(P2AM,-P2BA/IP2RL,*PBASPBASPCON#WPCON"APDMFPDMFPLOC, PLOC,PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLtSPACMSPACSPACMSVER4.0SVER 1SVER,,nScal@Scan3TUBEE1Tmpr<UserphARphCHphDYbigphQLcphTRphTR-"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikas1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqvLWLaU\,Z1@cMos5#|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#xg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhIxUKO.mQ;Rj:2`iDhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#RqAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTjjNUB1PtB1PtBCTS1BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &MDATA &M2ZDATA &MDATA &MDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab@BFVoc@1FWO_GATCFeat..@GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~0_LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM,&P1RL,$mP1WD,(P2AM,-P2BA/IP2RL,*PBASPBASPCON#WPCON"APDMFPDMFPLOC, PLOC,PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLtSPACMSPACSPACMSVER4.0SVER 1SVER,,nScal@Scan3TUBEE1Tmpr<UserphARphCHphDYbigphQLcphTRphTR