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***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5S^cdddddccccaa_^^]\ZYXVUSQONMKIHFECLLLDEE`aawww   ###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: cec42e3f642649289bd0be363abb9dcbSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-Well K1363.B7_psbAKB_3730_POP7_BDTv3.mobSeqPop7z=AFLTZ[XUU]m~vmaSE;658:83,$"2AJJ@1#".=HNNKKMRWWSMIIOW_a^TI>74332.'   %&%!"(-/+" 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"         NxLgyg`W< '06;>@A@<4( FsyO+ #{ :TP[$ +Z3-~@    )Kqv\C.9l ]| ln I  p #' *c-036.8:<>3?C???4>(<:8630-*&o" m ] l L!^0^u[G6&  "Q,'~zoe^\bnqgglsvupnr~vrt||bQD9-  torys]E1$"'/6850,,0586."&-,&%% &NsT=-#      !Gt N}1F 2] e.BLX=R(dg48Io(v<[r.eE (2-   #;Q_b\UWl7K + l ZdHRZ] S "   $adR+jO.zvvz~yqfXMHJS`nx}}ysnkjjjjigec_[WTSV]grz~zoe]UNO_  @uhoxgZV[fr{}|~}n\K>8:CMUUM?/$ %-6;;4*    S> ]i  K~5L 5+R    6eORNw-6H,C| | v{x[vx)c w+B$ V@>IXcdYD,    "XHy^T:36994/--.,&  <L|-^nNf-{N,      <9gc0G   #      4f)DZm{}j?vE*#'..(  I@puQO4,/7=<5,'.Iy)<+k0      ,a:8qF-%)6DMOJ@75?TpoT"2g1m)8@B>5* b$mDA@|hTD9312331.*)*.23/% (/0*! (/451%2Z)}N<@N[a]QB509QxxM1$%.9BGF>1!$M>G.s@"'-49;81''1893&2lR\M[x|X9.EJ6t:$+@Va^O:&"#"    "$     3sCS6L =kTH =Ah ?,;k+&@U^YJ6#     O<e]$g 1q"Z*t<.h$:}71j+ %OuI&  ;u0C4z9 '_ k7  .k7L: ~B.65+"MV+2YB @:\P>   +7;5* 3k [5 Et{P+O?jf4> ,BPRF2 4Xyb>  !&$    XqnHAQlp]MC>====?DKSY[YTNKLPW]a`[SJ@7, #ZxB]GGPWVMC<;=;/ >Iys9>+LHuko{iG!h*'V'bbk7!'Cnb%K\ZI1o/cPB4# -kbg#N*(6CLNJFJcvj +$E$P_mO I|cYqJ6110)'8BA5"             ")+($   -56."   $$ ")--'  (/10+# !$!                                                   (*&   "$# !(' +8><4+#!%)-13431112331.--/0.*# !  ##                                                                              ".560&   &397."#*,)!#(+,,+*)'%" "$                            #$              $!                                   (*$    '.0+##*.+#    $,+"   $.1.$      %),-..-+(&"*6AJORRQPLGB=;<BIQVYYWTPNLLMMJD;0'##&,020,'! %'&         ##                                 "                                               $"                                                                         $'#           "#                         !           t@=A9XAWgA2aKB.bcpKB_3730_POP7_BDTv3.mobtermAGGTGCCCTCTTGCGGCCCATCTTGCTAGCTGCTCTTGAGCTCATCTACAATGGATAAGACCTATTCTTAGTGTATGTGAGTTGAGGAAAAGAAAAAGTAGAAAAAATCCCCCCTTGGTCTATAAAAGGGGGCTTTATTTCGGAGAAATTTTCCCCCCTTGCTAGACCAATGCGGGATTTTTTCTACATTTATGTTTTTTAAAAAACTCTCATACACTAATAGATTGTTCTATCCATTAGTCCACGGTGGGGGCGGAGGTGCTCAATCTACTCGGGGTTATTATCATTGCGTTCATGTATAACCAAAAGGTGCCCTCTTGCGGCCCATCTTGCTAGCTGCTCTTGAGCTCATCTACAATGGATAAGACCTATTCTTAGTGTATGTGAGTTGAGGAAAAGAAAAAGTAGAAAAAATCCCCCCTTGGTCTATAAAAGGGGGCTTTATTTCGGAGAAATTTTCCCCCCTTGCTAGACCAATGCGGGATTTTTTCTACATTTATGTTTTTTAAAAAACTCTCATACACTAATAGATTGTTCTATCCATTAGTCCACGGTGGGGGCGGAGGTGCTCAATCTACTCGGGGTTATTATCATTGCGTTCATGTATAACCAAA .AQfq'5BRbs{!.7DN]iu$0;GR`kw &3IVdr{ #/9COXdp{%,3?KXdn{(4@KValx !.8HTamu   # , : F R ] h t    & 0 = H U a l z  " / : E Q \ h t     ! + 3 @ O V d m x  ( 3 = D O Z f l | $0: .AQfq'5BRbs{!.7DN]iu$0;GR`kw &3IVdr{ #/9COXdp{%,3?KXdn{(4@KValx !.8HTamu   # , : F R ] h t    & 0 = H U a l z  " / : E Q \ h t     ! + 3 @ O V d m x  ( 3 = D O Z f l | $0:   &   "2-+"$(,&# .++"$'+23 " "    ""2+1>;.1$-.11+1"       "                         ""              &   "2-+"$(,&# .++"$'+23 " "    ""2+1>;.1$-.11+1"       "                         ""           G% `HL &TB T2a+98(, Y;a5FJQ 9AS cP1O,7A#c\3<c-`]0>"2\P$="<]/.HP+-K_ ^A*!OXbUJ:0a*VVD9HG1,c9Z[0\"5WC-G>V8U1cFAVZ$ I$D0X0I]\^[2=SY P 15C;_9V7=a0#Z ,&H#!$Y+BO@J,Q$ -=PDS=\ X&B b "O^ MW=bb'LN(F/3J^;'2I 7''\.W5a: &3A//MS8 9<JrnV/Jpo; 8 SH GU&R\SnsKHDsE^ZH`cw i (%  j >V_4e&!= s  6 1_0 |#A -)HJW>N]i@@ V waXxi` cLiB[Z2vi_,(er[%^rT&g89:@vQk^ 7 v @ <qW`]!RN-nwMM::FSR\_|=*=BOA>$8532?*^OL\#OUd,v/ $eY, 8XJ|'%%=>s UQ<O_/&# "7N5T:&+[D?[0;:-k=( = } ' Fw4O.?>F= >B+A&3%BHe?p9p8< Tv5$p2"d|SbTBX*q tS]ZDmJ)+*yu_VgVVF%|DEx^YPR1(VU}()d=7Z+ 1k_H-/XpE$F<.EFEh$hAbM]e0C"84M- a~.QA7X8*BjjUphKoH/eyE`G$4M!v i/iU ]j\ &[!W f | O *`.a/zjt|2-&S;o-`  nb;#x$@|yat=&BT# _ 1"teQ8oFiO@91Vz$./tgCp 2tPR-@pH@S .><WaI.GpGATGTGATCTCGTGAAAAATATCAAAGTATGTTCTGCTCCGCGCAGACACTAATTATGATAAATCATCCTTGTCTAAACCCCCTTTAGGGCCCCTGTCAGTTTTCTTCTTTTGAAGTTCACCCTTTTAAAAAAAAAGAAAATCCTTTTAAAAGTTTAACAATATTGAACCGCGATAGAAAGAAACTGTCAATGCCCCCCCCCGGGGAAGATCGCACTAGGGTATAGAACTGTAATCAGTTGAGTTTCAACTTCAAAGTCAAGCACGGTGGGGGAAAACGTGGGGTGGGTATCCACCACGCGGGACGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:37 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $H(DATA $H(,DATA $H(tDATA $H(DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~FLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISJP1AM3f{P1RL3fP1WD3fP2AM3fP2BA33P2RL3fGPBAS33PBAS33}PCON33 PCON33 PDMFPDMFaPLOC3f IPLOC3fPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%BSCAN3SMED SMLtSMPLtSPACMSPACZSPACMSVER4.0SVER SVER,,nScal@Scan3TUBED9Tmpr<UserphARphCHxphDYbigphQLcphTRphTR/xThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $H(DATA $H(,DATA $H(tDATA $H(DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab,)FVoc,FWO_GATCFeat..+GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~FLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISJP1AM3f{P1RL3fP1WD3fP2AM3fP2BA33P2RL3fGPBAS33PBAS33}PCON33 PCON33 PDMFPDMFaPLOC3f IPLOC3fPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%BSCAN3SMED SMLtSMPLtSPACMSPACZSPACMSVER4.0SVER SVER,,nScal@Scan3TUBED9Tmpr<UserphARphCHxphDYbigphQLcphTRphTR@~A67OQ71{zK90plkm$48V^p$&:?wMB>-j%;U*E^h9i_Qw|k8 <.(P-S"EnKl/:5t9z"DEoh9ZzZW-W 7W}$wFTN]%jEX@u]YMAmB] ]ddc}PMfOl>o UekB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RUR6? sdWUW>|=)15]Mfk40H^p],!=%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tr%,yPyN+8ds16;evr./b?Ay0]:jjMSDDl[zL[I?pqOhv]dy%7qU+NU[xinJilZbCd]'p8Npdpu#Wc4hAC?-4$&FkNp&iJ-omMOxjYa/k#Pb~;H26t{_"#kI>b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme`T95\3&ML" 3]CUSt5R>9#\%EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMKLbsMvF(wD1RuM]4fMt}o].5sBc9yKy\rm#ulc*{d&]?#(eaK|`P@R(RlSR5y=Y`gXBY'^n7a;A{%i7#W yZE!e~8WYH|O[nYL.wJiBwH~?p MJM*{B]9[ikI0o6Gzv&1`Zlc*+?_u';fQGKerctp(kA;M4[!f<,hm<7eaAvG9 HtWo0>B|::zWT|O\rwHB*@Y%H_TQFGa^w^8/HAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTjj:<B1PtB1PtBCTSBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $H(DATA $H(,DATA $H(tDATA $H(DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab,)FVoc,FWO_GATCFeat..+GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~FLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISJP1AM3f{P1RL3fP1WD3fP2AM3fP2BA33P2RL3fGPBAS33PBAS33}PCON33 PCON33 PDMFPDMFaPLOC3f IPLOC3fPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%BSCAN3SMED SMLtSMPLtSPACMSPACZSPACMSVER4.0SVER SVER,,nScal@Scan3TUBED9Tmpr<UserphARphCHxphDYbigphQLcphTRphTR