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###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 43b89372883749258c325ad0c98dcd9dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-Well K1337.G8_psbAKB_3730_POP7_BDTv3.mobSeqPop7zRTX_iw  zoihigcZPHDEHLMLG@:52358:<=>@BEHLQU[bjs{zka[WRLB7-((-5=BC?9425<GUcq}3e 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MMMMNPTY`hosuttx 8X~:u8n=ks_G%r)tJ&/a`7,JnqMj63O2X! e<wFzrlg`UG9," *M{3s4QengO$\\%skijjhc]WSQRTUVVVWZ`eilmoqtz< q,KXQ8CK -N{3u(M]P$~g(|jZNEA??@ABFN^v }fRB6-'#"#%')*-8P||<j&myK/# $,5=CEB:0&#-J<D(_a,G3q=!%,241*  5Y?5 ;3vU, $*-.-.3@TnqT:&   /GcrV>,   /IguW<'    $FrxL+    'JrwZ?( &,//18E[xHOlPo. c / +-:&` d TB]aPh R&re[TNLLMNMHA92//38>CFHHGC=71--2:DMQQKC;77<CKPQNJGEGILNNNMNPSUWY\_cffc\UPPSZ_`\TKHM]u}naWPMLLLJGB;51..02431,&"  !           -b-QS2i2    )28>DLWfz%Q}=|c/f4^~rT*p,r8z2S!"[D[?c6$%.8?A=5+!!$%" ".7;5%Oek`*1?EB9-#"&'&"'.2/%HEDG!)*%     N_=|4  #   5ud{@+ J{g<"jDW|* *yJPG')$   1v'lu7; ()#   BoP'    )Ikc@!    :b[9  <ws:    _2Y@  %#   -LkjL1&.23459?GPYdq/HZ`YE%#K[L _3""   4k |A   AxJ.mU+ *O{S% #Sl=1]W)=r`7      -Vk=     +LliM4"   CqV+    /\l=     'Jo|Y5 ,8;3"BKcY0f3 !$"  An|O&3^sG$     'KnoN0     6[nH&     $Fk}X5 &'! +OueB#    0XvD$Td="  Dtg8 'Hk}^;     6PfrreO6  .RwgD%  '-,"P '}F !  #%"-PrwZ;#    #:RdmiZD+  8]uL$ 1`yS3!" )Hiy[9      ;Ysx`C'  !7LZ_XI4   /Kfy{hN4      " &KtZ6     )22' 3sF\K}<'T]=)"&/:?;/ <te9,FYywSM'"09;5*$&$!!*2782'5^|w&xc pHBZ?i{qOxYHBBEGIKLKGA7-#!$#                                                           oU@[A;AO=AKB.bcpKB_3730_POP7_BDTv3.mobtermCCCCCTTCCTCAAAACTTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAAATTGATTTTTTAGTTTAGTATATATGAGTTTTTTTTTTTTGAAAGTATAAGGGGCAATAAAAAATTTCTTGTTCGATCAAGAAACTAGTATTGCCCCTTTACTAGTTATTATTATTTTTTTTATTTCTTTACTTTTTTATTTCTTTAATATAAATCCTTAAAAAAAAAAAAATAAAACGACTTTTCAAAAGTCTTATTATGTAAAAAAATTTTTTTTTTTTTTTACTTTGAAAGGGGGCGGAAGTTGACTATTGGGGGAAGGTAGGGGATTGTGGTTCCTGCATGAACAAAGACCCCCTTCCTCAAAACTTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAAATTGATTTTTTAGTTTAGTATATATGAGTTTTTTTTTTTTGAAAGTATAAGGGGCAATAAAAAATTTCTTGTTCGATCAAGAAACTAGTATTGCCCCTTTACTAGTTATTATTATTTTTTTTATTTCTTTACTTTTTTATTTCTTTAATATAAATCCTTAAAAAAAAAAAAATAAAACGACTTTTCAAAAGTCTTATTATGTAAAAAAATTTTTTTTTTTTTTTACTTTGAAAGGGGGCGGAAGTTGACTATTGGGGGAAGGTAGGGGATTGTGGTTCCTGCATGAACAAAGA$,2;EX\sx)6BQar} +8@LWbmw &/>IS^hu!-9DR]gq~  ,8DP[er~&1=IS_kw +5CO\fq~$1<IUamy  " / 8 A L [ f s     & 3 > K V a l w   - 8 D R [ h u    & 2 ? K W c p |  ! - 9 E Q ] j v +7CN[gs(1=KWcmy$,2;EX\sx)6BQar} +8@LWbmw &/>IS^hu!-9DR]gq~  ,8DP[er~&1=IS_kw +5CO\fq~$1<IUamy  " / 8 A L [ f s     & 3 > K V a l w   - 8 D R [ h u    & 2 ? K W c p |  ! - 9 E Q ] j v +7CN[gs(1=KWcmy  ( %2   &2#.&14->88883' '*'3+)++$33+;,.3 38)"&".2.06;1)+  ""+("'$"""  " "       ""($""        "   "&         ( %2   &2#.&14->88883' '*'3+)++$33+;,.3 38)"&".2.06;1)+  ""+("'$"""  " "       ""($""        "   "&       +LGE*B#2$RBV*)'$\-Y@\5"G&?%L.$1QMSJTI#0IOW@J *T LDN] b,_P6S47H[%.L#)/[;=CX2@^5&\<T!,2OKENA 6\6S GG^@ [; VH0;+F<'\1b4 L60'I#?X6^)b4I,%*I?=8Z4[6>@\+M3aM7X9H\c F1L;,cLU?DZ>=33T%Z?DPF" 4NQ/!R0:RS LQHC 8BKaMKLAZ0I\BKDN5WR*_K*B'8QA/Y68 bM_VB/61 8(44yCe<vN{UwkNZt&p.AIm3v9N=SIP3P   [{,;0^w^  HV  -m)HY6 |K~CsV,0  pwM(,9#%Z{jd0AL.FRgSf!.gS&O{9ri Uc@PM !pDn'P}|mQE!O_vW3b  w$Z`A\/iezdwV`eEF`@Man$ !t ) 0h RK? `!!Dg # x7\9qfDE:Pa-(/!L6(?-&D( ,h-!\PK =+' v :b(.  "2a&*`,:K $)F#s ;8p{\)F+}dg?W?kVNp|8Uj: :=^Du*W66{ s-C+c=A=wB B^_BnILq;,SNPb5O1%J9?;CB2WGoP5Q>M@i-994n n- |-aJdR/D0*]8yaCSpC/)jp RZ xV\9{6g"6^>G\EC An]Us"y/(   T4O9h]su<dr F 5 \ZF>O:TXh3Yl[OgQQ_SM5p$R B,?PafVo.ka8c<iH1Am N<$3>MfUPfYGj5qe.1RK8Q^QI;C#;@U1Frgb7RZ +vhuX}LE}Eq[=}U52L9AAAAAAATTCTTTGCACATATGCGGCGCCCTGCTACATAGACTCGTATATGGCAGATACCCGGTAAAATAAGACCCCTTTAAAGGCGCCCAATTTTTATATTTTTCAGGGTTTTCCCCCTAAAAATATAACGATTCAGGAATAAAAAAAAAATTATAACCCAGGAGAGGGGCAATACAAGGATTCGGAACGACCAAAAGCATATTTATTGCCCCTTTTCCTTTCAGGGCAAGAAACATGTTTACACCTAACGAACCGGTCGACGGAAACCAACGGAGGAAGAGCCTTCAAGAACTTCAACGACGAAAATCGGATCTTTAACAGTACAACTAACGCGACCAGGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:13 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtyASPtyB1PtxB1PtxBCTS9BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA '7NnDATA '7Nn3DATA '7Nn~DATA '7NnDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~80LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISbP1AMY,P1RLY)]P1WDY.P2AMY4%P2BAYY6P2RLY1sPBASYYPBASYYPCONYY(PCONYY&PDMFPDMFyPLOCY#PLOCY!GPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%ZSCAN3SMED SMLtSMPLtSPACMSPACrSPACMSVER4.0SVER 9SVER,,nScal@Scan3TUBEC4Tmpr<UserphARphCHphDYbigphQLcphTRphTR#This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtyASPtyB1PtxB1PtxBCTS9BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA '7NnDATA '7Nn3DATA '7Nn~DATA '7NnDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabHFVocHFWO_GATCFeat..GGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~80LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISbP1AMY,P1RLY)]P1WDY.P2AMY4%P2BAYY6P2RLY1sPBASYYPBASYYPCONYY(PCONYY&PDMFPDMFyPLOCY#PLOCY!GPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%ZSCAN3SMED SMLtSMPLtSPACMSPACrSPACMSVER4.0SVER 9SVER,,nScal@Scan3TUBEC4Tmpr<UserphARphCHphDYbigphQLcphTRphTRV"O`x83E> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W+!'eAbCu]^H98$L?% .9\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvU&Wy+W+$8N6!@_{,? 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