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(                                                                                                                                                        d,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5S^cdddddccccaa_^^]\ZYXVUSQONMKIHFECLLLDEE`aawww   ###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5c555f4b3c57483fb49ff44ae9072bf7Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-WellK1336.F11_psbAKB_3730_POP7_BDTv3.mobSeqPop7z----./149@JT]dhihhhkr|vkaYTQQQPNKIGFGHHE?7.'" 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ZWsa*Z4"#(+-+& 4FOL=+'>YmsgL+5YwvY9CuX3       ,IdtwkU=' ,:DHE<.  !5GSVQD3"   "Jy}L!")(   'Qp0__}k0= 0;=6+ &/8>?9- 5_rP6$!%%"   )D_u~xeH* K# L#    (02-$    8eyH       ;pk7    'Oa6       9p%a4%8h%gPFC@:1**5Id}lO6('5Ley{hO5   (>R]]Q>)      *UmF&)./*" Azc=' !" &19<7,(Q /8 q= #(*& ?qhG- ,8<7)+Pxe@! #&! +362*(>P[ZN;%,If}qY>'   (KqcD,      -@OVSF4  ,461'   )X}J#   #*,*"   +] \A3//120+$ )5>@:/ 1d@MB"kS=,"$(*)%!!)<XwdC'2d_fYB940+'',3;><60/6EXhrqeS@2+,28;80($-DhV!]g! |aygK7-'$""#'-253-'""&+//)                                                                                                                                                                                                              *@ߖSA 0UAOyAjKB.bcpKB_3730_POP7_BDTv3.mobtermAACCATTCGTTAGACTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAGACTTCCGTCTTAATGTATTGTATGAACAATTATTGAAGGAGCTATACCCCATATCTTTTCCAAGAGGAAAGGAATAGAAAATTCAAAAATGGAAAATAAAAAAAAGGAGTAATCAACTGTGACACGTTCACTAAAAAAAATTCCCACACATTTCATTCATTGGCGGGGGATAACGGCAAAGAAGGAAGCTATACCCCATATCTTGGAAAAGATATGGGGTATAGCTCCTTCAATAATTGTTAATACAATACATTAAGACGGAAGTCTTATCCATTTGTAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGAATTACGTTCATGCATAAACAAAGAAACCATTCGTTAGACTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAGACTTCCGTCTTAATGTATTGTATGAACAATTATTGAAGGAGCTATACCCCATATCTTTTCCAAGAGGAAAGGAATAGAAAATTCAAAAATGGAAAATAAAAAAAAGGAGTAATCAACTGTGACACGTTCACTAAAAAAAATTCCCACACATTTCATTCATTGGCGGGGGATAACGGCAAAGAAGGAAGCTATACCCCATATCTTGGAAAAGATATGGGGTATAGCTCCTTCAATAATTGTTAATACAATACATTAAGACGGAAGTCTTATCCATTTGTAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGAATTACGTTCATGCATAAACAAAGA'0JRXgr|+9ESct~.:BOZht'4>JYfq{ "/<GS]it'3?HT_l} !-8EQ[fr~ ".:EQ^kx*4AO\hq}   , 7 C N ] j v    + 8 F V f p |    $ 0 < G S ] i u    ( 5 A J V a n {  $ / : E R \ h t  #1<GUbmz !-9FQ\iv)5@MZer(5AMZdp} $/;HT`lx'0JRXgr|+9ESct~.:BOZht'4>JYfq{ "/<GS]it'3?HT_l} !-8EQ[fr~ ".:EQ^kx*4AO\hq}   , 7 C N ] j v    + 8 F V f p |    $ 0 < G S ] i u    ( 5 A J V a n {  $ / : E R \ h t  #1<GUbmz !-9FQ\iv)5@MZer(5AMZdp} $/;HT`lx   + ( "((,  #& (,$,   '    "!                ++#"   #  "$"  01"  ".++(# "&".2'$"  " #"! 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"    "' 1   # +&  " ""  ),8R: /'W]M$T#O2a0$HI%NLD8AK <(CXVQPGM7-4,^6A7_Z5X`2*LC&!9[HI14 &Z!I.Y!( I_+SO@^4Q_9!(9 "/'DNF6LK #1`)2>7>!8dQ R<a8PI[O_C6LcUC>F13H`MQ>2M 2`*6KT<>[,+!_#8D"2!Y;,= +@C!<`dC ) 4*<>c =6!)D #WS&aW( Ka#V&H 0ZD52QQ%N%]ASDTW>2OG`= H` %&'QOK1 5Y$"GbSI)Y PE_W_[B9:Z3R7` bN5U=6S, 9)EYM7,?Q9%8J$+R( y#L1g%y*-uFA/Z#F#-1]yZB) " w}fQA[ #a&>/>(pk(hiT#  [ BEh2vDwPLd,:uFA$F&$hF# ? ^ yWk^6<v.#A&m _ / RitggM%>!Wv`*L_qetHj}}4]`cMHV- 7267Nsw9]<&",1g=;H, TT<  y+cG]:F o aHn_g}k7A\PKS AQm +qr$98 u@rAi~HT[]niaY::\J Q2".#N#/ .(] $  &:5   F!  >3!p%9pZH AA7 0d?*1{=J} ; ?,SupW/C{gcEpIql'p*CWE|wD%u#%88?oi_'X{CTqQ8Gb 6cC(8z@FQK ,G |}Vmpx%Ja9!-nR-uLLTy=gB5?#f{EIl88YI`@%x S0MuoZ]MZ) 0 l%hYpU1 YJzo`[d}L"Ib0 WT!/LGm[6EuJuf^=nf745?NB 0~)N>VfE,<_I^a{%~4F1gZ XB|6CCAACCCTCCCTACAATATGCGGCGCCCTGCAACCAGCACTTGAATATTCCTCCTTTCTCATTCTGCAGTCCCCCTTTTAAGGCCCCCTTCAATCTTAGATCGAGAAAATACCCCCTTTTAATGATTCTTCCCTCTTTGCCTGTTTACCCCCGCCCATTATTGGCCAGTGTCTCTTCCTTTATTGTGGTCCCTTTTAGATGAAGCACACCCCTTTTTTTTATTTACTTTTTTGAATTTTACACAAATCCCCAAGAAGGTCTCGCGATCTAGGTATGAACGTCCGTTAAAACTCACTCCATGGAGTGAGGAAGGAAGAGTAAATCCCTAGTCGATGTAACGGGAGTATCTAGCCAAGTAGATCGGAGCAGAGTCTCGTTAACTCACACCGCCAGGGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:12 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTS<BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'N.DATA 'N.2DATA 'N.DATA 'N.-DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~; LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIScP1AM-P1RL)P1WD02P2AM6fP2BA9P2RL3LPBAS#PBASPCON(qPCON&PDMFPDMFzPLOC#PLOC PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%[SCAN3SMED SMLtSMPLtSPACAnSPACsSPACAnSVER4.0SVER <SVER,,nScal@Scan3TUBEC3Tmpr<UserphARphCHphDYbigphQLcphTRphTR#%This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTS<BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'N.DATA 'N.2DATA 'N.DATA 'N.-DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabJFVocJFWO_GATCFeat//JGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~; LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIScP1AM-P1RL)P1WD02P2AM6fP2BA9P2RL3LPBAS#PBASPCON(qPCON&PDMFPDMFzPLOC#PLOC PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%[SCAN3SMED SMLtSMPLtSPACAnSPACsSPACAnSVER4.0SVER <SVER,,nScal@Scan3TUBEC3Tmpr<UserphARphCHphDYbigphQLcphTRphTRYxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["[87tF4WxS5h#@lCI|fl9g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLV>BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|$q_fUG9bUSZa2Ur4_^+\Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? M;8'i2{ysPl`A(.:U q<6(mu y-WPv8U)HJxmAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt}ASPt}AUDTllYB1Pt|B1Pt|BCTS<BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'N.DATA 'N.2DATA 'N.DATA 'N.-DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabJFVocJFWO_GATCFeat//JGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~; LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIScP1AM-P1RL)P1WD02P2AM6fP2BA9P2RL3LPBAS#PBASPCON(qPCON&PDMFPDMFzPLOC#PLOC PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%[SCAN3SMED SMLtSMPLtSPACAnSPACsSPACAnSVER4.0SVER <SVER,,nScal@Scan3TUBEC3Tmpr<UserphARphCHphDYbigphQLcphTRphTR